Research Article
Volume 5 Issue 9 - 2021
Biofilm Formation by Staphylococcus aureus and Escherichia coli Isolated from Patients with Suspected Nosocomial Infections and their Association with Antibiotics Resistance
Eidha Ali Bin-Hameed1*, Amal Ali Bahakim2 and Maryam Hamed Baras3
1Biology Department, Faculty of Sciences, Hadhramout University, and Health Sciences Department, Faculty of Medicine and Health Sciences, University of Sciences and Technology, Hadhramout, Yemen
2Biology Department, Faculty of Sciences, Hadhramout University, Yemen
3Biology Department, Faculty of Science, Hadhramout University and Molecular Biology Diagnostic Unit, National Center of Public Health Laboratories, Hadhramout Coast, Yemen
*Corresponding Author: Eidha Ali Bin-Hameed, Biology Department, Faculty of Sciences, Hadhramout University, Yemen.
Received: July 02, 2021; Published: August 30, 2021




Abstract

Background: Bacteria attach to surfaces and produce polysaccharides resulting in the formation of biofilms which are involved in a wide range of human nosocomial infections such as urinary tract infections (UTIs) and surgical sites infections (SSIs). Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) are the most common bacteria causing nosocomial infections leading to serious health issues. Bacterial biofilms are considered to be highly resistant to antimicrobial agents.

Objectives: This study was aimed to performing in vitro detection of biofilm formation of S. aureus and E. coli strains isolated from patients with suspected nosocomial UTIs and SSIs and determine their susceptibility patterns to antibiotics in Mukalla city, Hadhramaut, Yemen during the period from December 2018 to May 2019.

Methodology: A total of 60 clinical isolates of S. aureus and E. coli were isolated and identified by standard bacteriological procedures, then subjected to biofilm detection by tissue culture plate (TCP) method. The antibiotics susceptibility test was performed by Kirby-Bauer disc diffusion method. Chi‑square test was used to assess the statistical significance of observed antibiotic sensitivity. A value of P < 0.05 was taken as significant.

Results: Of the total clinical isolates of S. aureus and E. coli, TCP method detected 33(55%) as strong, 15 (25%) as moderate and 12 (25%) as weak/non-biofilm producers. Biofilm forming S. aureus developed significantly higher degree of resistance towards antibiotics drugs for amoxiclav 100%, ceftazidime 95.8%, cefotaxime 62.5%, cefadroxil 45.8%, ciprofloxacin 41.7% and ceftriaxone 25% with statistically significant correlation of amoxycillin/clavulanic acid and ceftazidime resistance and bacterial biofilm production (P-value < 0.05). The rate of antibiotic resistance biofilm forming E. coli were 100% for amoxiclav, cefadroxil 91.7%, cefotaxime 75%, ceftazidime 70.8%, ceftriaxone 66.7%, ciprofloxacin 62.5% and co-trimoxazole 33.3% with statistically significant correlation of cefadroxil resistance and bacterial biofilm production (P-value < 0.05).

Conclusion: TCP method showed that S. aureus and E. coli isolated from suspected nosocomial UTIs and SSIs have high degree of biofilm forming ability. A high antibiotics resistance and multi-drug resistance was observed in biofilm producers than non-biofilm producers.

Recommendation: Of recommended antibiotics therapies for UTIs and SSIs, amoxycillin/clavulanic acid, cefadroxil, cefotaxime and ceftazidime were the least active antibiotics, whereas co-trimoxazole and amikacin were found as the most effectual for S. aureus and E. coli biofilm producer.

Keywords: Biofilm; Escherichia coli; Multi-Drug Resistance; Staphylococcus aureus; Tissue Culture Plate

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Citation: Eidha Ali Bin-Hameed., et al. “Biofilm Formation by Staphylococcus aureus and Escherichia coli Isolated from Patients with Suspected Nosocomial Infections and their Association with Antibiotics Resistance”. EC Emergency Medicine and Critical Care 5.9 (2021): 39-46.

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