Review Article
Volume 5 Issue 7 - 2021
Neurodegeneration and Cytomegalovirus
Ibekwe RU*
Biology Department, Texas Woman’s University, Texas, United States
*Corresponding Author: Ibekwe RU, Biology Department, Texas Woman’s University, Texas, United States.
Received: June 03, 2021; Published: June 30, 2021




Abstract

Introduction: Alzheimer’s disease (AD) is the most frequent cause of dementia in the elderly, and it is characterized by extracellular amyloid plaques and intracellular neurofibrillary tangles (NFTs). Several studies have shown clinical correlation between various herpes viruses and neurodegeneration (ND), others have demonstrated coincident markers for AD and Herpes Simplex Virus-1 (HSV-1) infection, but there is yet no study investigating Cytomegalovirus (CMV) and AD.

Human Cytomegalovirus (HCMV) is specie specific, and the scope of mechanistic study is limited; however, because of great similarities in genetics and pathology between HCMV and Murine Cytomegalovirus (MCMV), MCMV will be used as a model system for investigating ND in mice and the result obtained extrapolated to humans.

The study will be carried out to determine the presence of neurodegenerative markers (Aβ42 and high molecular weight tau) in IC-21 macrophage cells and in primary microglial cells following infection with MCMV.

Methods: IC-21 macrophage, 5 x 105 cells will be seeded unto 60 mm dishes and infected 24 hours later with MCMV at a multiplicity of infection (MOI) of 2 plaques forming unit/cell. Cell lysates will be harvested at different time points post infection. Total protein will be quantified, and 20 µg protein loaded into each lane of SDS-PAGE and electrophoresis carried out to separate the proteins. Separated proteins will be transferred to membrane and probed with rabbit polyclonal anti-Aβ42, rabbit polyclonal anti-tau and mouse monoclonal anti-β actin will be the loading control. Indirect immunofluorescence microscopy will be used to detect the presence of the protein of interest. Primary microglial cells will be isolated from P2 brains of mice and homogenized in 2 ml TS buffer (50 mM Tris-HCL) allowing to stabilize. MCMV infection will be done on day 15. Microglial cells will be disrupted with sonicator and centrifuged at 300,000g (450,000 rpm) for 30 minutes.

Conclusion: Presence of high molecular weight tau and Aβ42 following infection of the cells with CMV shows that CMV which belongs to the same family (Herpesviridae) like HSV-1 also induces neurodegeneration.

Keywords: Neurodegeneration; Alzheimer’s: Cytomegalovirus

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Citation: Ibekwe RU. “Neurodegeneration and Cytomegalovirus”. EC Emergency Medicine and Critical Care 5.7 (2021): 118-124.

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