Research Article
Volume 4 Issue 10 - 2020
Echium angustifolium Reduces/Revokes the Effect of Cerastes cerastes Vesnom in Albino Mice
Inass A Sadawe1, Nisreen H Meiqal1, Amira A Gbaj2, Salah M Bensaber1, Abdulathim A A Alshoushan3, Halima A Gbaj1, Jamal A Elbakay1, Massaud Salem Maamar4, Anton Hermann5 and Abdul M Gbaj1*
1Biochemistry Department, School of Pharmacy, Istanbul Medipol University, Turkey
2Novelien School, Tripoli Centre, Tripoli, Libya
3Food and Drug Control Centre (LFDA), Tripoli, Libya
4Zoology Department, Faculty of Science, Tripoli University, Libya
5Department of Biosciences, University of Salzburg, Salzburg, Austria
*Corresponding Author: Abdul M Gbaj, Professor of Genetics and Biochemistry, Department of Medicinal Chemistry, Faculty of Pharmacy, University of Tripoli, Libya.
Received: September 11, 2020; Published: September 28, 2020




Abstract

Snakebites are a tremendous challenge in public health owing to their high mortality. Most snake venoms cause powerful local tissue damage leading to transitional or permanent disability of victims. The anti-Cerastes cerastes venom effect of aqueous aerial parts of Echium angustifolium extract was explored using molecular modelling and animal models. The improvement in the mean survival time of albino mice was used to construe the antivenom property of this extract after challenging with LD99 snake venom. Echium angustifolium aqueous extract constituents (pyrrolizidine alkaloids, phenolic acid derivatives and flavonoids) showed van der Waals, hydrogen bonding and electrostatic interactions with an active site of the main constituents of the venom phospholipases A2 enzyme and changes in the fluorescent spectrum of the enzyme. The aqueous extract of Echium angustifolium extract applied intraperitoneally considerably increased the mean survival time but could not protect animals from death when used alone. The lower dose of 0.5 ml Echium angustifolium extract (1.29g/30 ml) showed less protective activity of 8.5 hours in comparison to the higher dose 2.0 ml (1.29g/30 ml) which gave a 13 hour protection time. The anti-snake venom was found to be more efficient than the aqueous aerial parts of Echium angustifolium extract.

Keywords: Cerastes cerastes; Anti-Snake Venom; Aqueous Echium angustifolium; Molecular Modelling/Modelling

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Citation: Abdul M Gbaj., et al. “Echium angustifolium Reduces/Revokes the Effect of Cerastes cerastes Venom in Albino Mice”. EC Emergency Medicine and Critical Care 4.10 (2020): 74-88.

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