Research Article
Volume 4 Issue 7 - 2020
In Vitro Antiplatelet Aggregation Activity of Aqueous and Alcoholic
Leaf Extracts of Azadirachta indica
Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, Tamilnadu, India
*Corresponding Author: Sankaranarayanan Chandrasekaran, Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, Tamilnadu, India.
Received: April 15, 2020; Published: June 30, 2020
Abstract
Azadirachta indica considered as 'kalpavriksh of kalyuga’ has diverse pharmacological properties. The present study was carried out to assess the in vitro antiplatelet aggregation activity of Azadirachta indica leaf extracts by platelet aggregation assay using platelet rich plasma (PRP). Aqueous and alcoholic leaf extracts (AILE) were prepared and the percentage inhibition of platelet aggregation was examined and compared with standard drug aspirin. The studies revealed that both the extracts dose dependently inhibited platelet aggregation. The effect was more pronounced in alcoholic extract (45.8%) than aqueous extracts (25%). These results suggest that phytoconstituents of leaf extract exerted antiplatelet aggregation activity. However further studies to identify the active ingredient and their molecular mechanism needs to be explored.
Keywords: Azadirachta indica; AILE; Antiplatelet Aggregation Activity; Platelet Rich Plasma
References
- Amidi, et al. “Electrochemical synthesis of novel 1, 3-Indandione derivatives and evaluation of their antiplatelet aggregation activities”. Iranian Journal of Pharmacological Research 12 (2013): 91-103.
- Steinhubl SR., et al. “Early and sustained dual oral antiplatelet therapy following percutaneous coronary intervention: a randomized controlled trial”. Journal of the American Medical Association19 (2002): 2411-2420.
- Jura-Szołtys E and Chudek J. “Epistaxis as the reason for premature discontinuation of clopidogrel after percutaneous coronary angioplasty with stent implantation”. Kardiologia Polska8 (2011): 817-823.
- Quraishi HA., et al. “Therapeutical and medicinal properties of Neem (Azadirachta indica) in context of Unani system of medicine: A review study”. Journal of Drug Delivery and Therapeutics6s (2018): 394-399.
- Naidu J., et al. “Antiplatelet activity and quantification of polyphenols content of methanol extracts of Ocimum basilicum and Mentha spicata”. Research Journal of Pharmaceutical, Biological and Chemical Sciences5 (2015): 1236-1244.
- Vidhya U., et al. “In vitro anti-platelet aggregation activity of a classical hridya (Cardioprotective) yoga of bhavamisra”. World Journal of Pharmacy and Pharmaceutical Sciences1 (2016): 1310-1317.
- Prashanth GK, Krishnaiah GM. “Chemical composition of the leaves of Azadirachta indica Linn (Neem)”. International Journal of Advancement in Engineering Technology Management and Applied Science 1 (2014): 22-31.
- Lestari S., et al. “In vitro antiplatelet aggregation activity of Centella asiatica (L.) urban ethanolic extract”. International Journal of Pharmaceutical and Clinical Research 8 (2016): 280-283.
Citation: Lalitha Chandrasekar and Sankaranarayanan Chandrasekaran. “In Vitro Antiplatelet Aggregation Activity of Aqueous and Alcoholic Leaf Extracts of Azadirachta indica". EC Diabetes and Metabolic Research 4.7 (2020): 13-17.
Copyright: © 2020 Lalitha Chandrasekar and Sankaranarayanan Chandrasekaran. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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