Research Article
Volume 19 Issue 10 - 2020
Effect of Different Chelating Agents on Calcium Ion Quantification and on Microhardness of Human Radicular Dentin-An In Vitro Study
Ashish Jain1, P Karunakar2, Umrana Faizuddin3, Raji Viola Solomon3*, Marupaka Rasagna4 and P Shanti Priya5
1Reader, Department of Conservative Dentistry and Endodontics, KNR University, India
2Professor and Head of the Department of Conservative Dentistry and Endodontics, KNR University, India
3Professor, Department of Conservative Dentistry and Endodontics, KNR University, India
4Post Graduate Student, Department of Conservative Dentistry and Endodontics, KNR University, India
5Senior Lecturer, Department of Conservative Dentistry and Endodontics, KNR University, India
*Corresponding Author: Raji Viola Solomon, Professor, Department of Conservative Dentistry and Endodontics, Panineeya Institute of Dental Sciences and Research Centre, Hyderabad, Telangana, India.
Received: May 21, 2020; Published: October 27, 2020




Abstract

Introduction: The purpose of this study was to evaluate the effects of 17% ethylenediaminetetraacetic acid, 1% Phytic acid, 0.2% Chitosan on Calcium ion quantification and microhardness of human root canal dentin using inductively coupled plasma optical emission spectrometry and Vickers microhardness tester.

Materials and Methods: A total of eighty extracted mandibular premolars were decoronated and root canal treatment was prepared. Samples were divided into two subgroups, one half for calcium ion quantification and other half for microhardness testing. Based on the type of chelating agent used in each subgroup, samples (N = 10) were divided Repetition (three test and one control). The calcium loss was evaluated using the inductively coupled plasma optical emission spectrometry and microhardness with Vickers microhardness tester.

Statistical analysis: Data were analyzed for calcium ion quantification using one-way ANOVA, Post hoc Tukey’s test and for microhardness testing using Student’s t test.

Results: Calcium loss along with resultant drop in microhardness were maximal in ethylenediaminetetraacetic acid (EDTA) group with a significant difference from Phytic acid and Chitosan group (p < 0.05). There was no statistically significant difference seen between the Phytic acid and Chitosan groups (p > 0.05) in both the tested parameters (Calcium ion quantification and Microhardness).

Conclusion: Use of 0.2% chitosan and 1% phytic acid can be thought of as alternatives to ethylenediaminetetraacetic acid when used as chelating agent when used along with sodium hypochlorite.

Keywords: Calcium; Microhardness; EDTA; Phytic Acid; Chitosan; ICP-OES; Vickers Microhardness Tester

References

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Citation: Raji Viola Solomon., et al. “Effect of Different Chelating Agents on Calcium Ion Quantification and on Microhardness of Human Radicular Dentin-An In Vitro Study”. EC Dental Science 19.10 (2020): 83-95.

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