Research Article
Volume 19 Issue 8 - 2020
Transfection of Human Gingival Fibroblasts with Cementum Protein 1 does not Alter the Ultrastructure of the Interchromatin Granule Clusters in the Cell Nucleus
Edith Florely Del Toro-Rangel1, Reyna Lara-Martínez1, María de Lourdes Segura-Valdez1, Higinio Arzate2, Carlos Esteban Villegas-Mercado3 and Luis F Jiménez-García1*
1Electron Microscopy Laboratory and Cell Nanobiology Laboratory, Department of Cell Biology, Faculty of Sciences, National Autonomous University of Mexico (UNAM), Circuito Exterior, Mexico
2Faculty of Dentistry, Periodontal Biology Department, DEPeI, National Autonomous University of Mexico (UNAM) Mexico City, Mexico
3Unidad de Posgrado, Facultad de Odontología, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México
*Corresponding Author: Luis F Jiménez-García, Electron Microscopy Laboratory and Cell Nanobiology Laboratory, Department of Cell Biology, Faculty of Sciences, National Autonomous University of Mexico (UNAM), Circuito Exterior, Mexico.
Received: July 17, 2020; Published: July 29, 2020


The cell nucleus contains domains related to gene expression. The nucleolus is involved in pre-rRNA metabolism while interchromatin granules are involved in pre-mRNA metabolism. It was recently shown that no ultrastructural changes are detected in the nucleolar organization, but no data of the effect of transfection in speckles are known. The aim of this work is to know if the interchromatin granule clusters display ultrastructural changes when introducing cement protein 1 (CEMP1) genes into human gingival fibroblasts (HGFs), a cell line of interest since they do not mineralize and upon transfection they perform mineralization, a potential to use in treatments of periodontal disease. Here we explored ultrastructural changes in interchromatin granule clusters after transfection of CEMP1. Non transfected and transfected cells display similar nuclear particles that form clumps in the interchromatin space and contain 10 - 15 nm in diameter individual granules intermingled with fibers, which are composed of ribonucleoproteins. We conclude that they correspond to interchromatin granule clusters. The absence of ultrastructural changes in the morphology of interchromatin granule clusters suggests that after transfection the general ultrastructure of particles related to pre-mRNA metabolism is not altered. These data may support the development of new dental materials.

Keywords: Cementum Protein 1; Human Gingival Fibroblasts; Interchromatin Granule Clusters; Cell Nucleus; Transfection


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Citation: Luis F Jiménez-García., et al. “Transfection of Human Gingival Fibroblasts with Cementum Protein 1 does not Alter the Ultrastructure of the Interchromatin Granule Clusters in the Cell Nucleus”. EC Dental Science 19.8 (2020): 179-186.

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