Research Article
Volume 19 Issue 2 - 2020
Comparative Evaluation of Using Leukocyte-Platelet Rich Fibrin (L-PRF) and Emdogain (EMD) in Guided Tissue Regeneration (GTR) Technique for Reconstruction of Periodontal Intrabony Defects in Patients with Periodontitis
Janet Moradi Haghgou, Erfan Abbasi Atibeh, Alireza Zamani, Ghodratollah Roshanaei and Nazli Rabienejad*
Department of Periodontology, Hamadan University of Medical Sciences, Iran
*Corresponding Author: Nazli Rabienejad, Department of Periodontology, Hamadan University of Medical Sciences, Iran.
Received: November 11, 2019; Published: January 29, 2020




Abstract

Background: Positive results of using GTR technique for reconstruction of intrabony defects which have been proven in many studies. Aim of this study was to evaluate the effect of using L-PRF and EMD growth factors along with GTR technique for reconstruction of periodontal intrabony defects.

Methods and Materials: Forty five intrabony defects resulted from periodontal disease which can be reconstructed were enrolled in the study. Defects divided into three groups by simple sampling (n = 15). Group 1 just got GTR treatment as control group, group 2 got L-PRF along with GTR treatment and in group 3 EMD used along with GTR. Clinical factors such as periodontal probing depth (PPD), clinical attachment level (CAL), keratinized gingival width (KGW) and bleeding on probing (BOP) were evaluated before and six months after treatment. Also amount of bone fill was evaluated with standard radiography.

Result: Treatment in all three groups significantly result in reduction of PPD and gaining of CAL. Sites with BOP significantly decreased, defects significantly filled with bone, however, there was no significant difference in KGW. Intragroup comparison showed no significant difference among groups in evaluated factors. But the most PPD reduction was 4.5 ± 1 in L-PRF+GTR group, the most CAL gain was 3.73 ± 2 in GTR group, the most reduction in BOP sites were 14 sites in L-PRF+GTR groups and the most bone fill was 73% in GTR group.

Conclusion: Using EMD or L-PRF along GTR treatment does not have additional effect on positive results of GTR treatment alone.

Keywords: GTR; L-PRF; EMD; Periodontal Probing Depth; Clinical Attachment Level

References

  1. Esposito M., et al. “Enamel matrix derivative (Emdogain(R)) for periodontal tissue regeneration in intrabony defects”. The Cochrane Database of Systematic Reviews 4 (2009): CD003875.
  2. Gupta SJ., et al. “Efficacy of platelet-rich fibrin vs. enamel matrix derivative in the treatment of periodontal intrabony defects: a clinical and cone beam computed tomography study”. Journal of the International Academy of Periodontology 16.3 (2014): 86-96.
  3. Pietruska M., et al. “Four-year results following treatment of intrabony periodontal defects with an enamel matrix derivative alone or combined with a biphasic calcium phosphate”. Clinical Oral Investigations 16.4 (2012): 1191-1197.
  4. Femminella B., et al. “Clinical Comparison of Platelet-Rich Fibrin and a Gelatin Sponge in the Management of Palatal Wounds After Epithelialized Free Gingival Graft Harvest: A Randomized Clinical Trial”. Journal of Periodontology 87.2 (2016): 103-113.
  5. Ajwani H., et al. “Comparative evaluation of platelet-rich fibrin biomaterial and open flap debridement in the treatment of two and three wall intrabony defects”. Journal of International Oral Health: JIOH 7.4 (2015): 32-37.
  6. Arweiler NB., et al. “Antibacterial effect of an enamel matrix protein derivative on in vivo dental biofilm vitality”. Clinical Oral Investigations 6.4 (2002): 205-209.
  7. Sculean A., et al. “Treatment of intrabony defects with enamel matrix proteins and guided tissue regeneration. A prospective controlled clinical study”. Journal of Clinical Periodontology 28.5 (2001): 397-403.
  8. Mitani A., et al. “Five-year clinical results for treatment of intrabony defects with EMD, guided tissue regeneration and open-flap debridement: a case series”. Journal of Periodontal Research 50.1 (2015): 123-130.
  9. Jian CX., et al. “Effects ofrhBMP-2gene transfection to periodontal ligament cells on osteogenesis”. Bioscience Reports (2017).
  10. Kanoriya D., et al. “Synergistic Approach Using Platelet-Rich Fibrin and 1% Alendronate for Intrabony Defect Treatment in Chronic Periodontitis: A Randomized Clinical Trial”. Journal of Periodontology 87.12 (2016): 1427-1435.
  11. Choukroun J., et al. “An opportunite’ in paro-implantology: The PRF (in French)”. Implantodontie 42 (2000): 55-62.
  12. Kobayashi M., et al. “A proposed protocol for the standardized preparation of PRF membranes for clinical use”. Biologicals 40.5 (2012): 323-329.
  13. Fujioka-Kobayashi M., et al. “Optimized Platelet-Rich Fibrin With the Low-Speed Concept: Growth Factor Release, Biocompatibility, and Cellular Response”. Journal of Periodontology 88.1 (2017): 112-121.
  14. Dori F. “[Effect of combined therapeutic methods on healing of periodontal vertical bone defects in regenerative surgery]”. Orvosi Hetilap 150.11 (2009): 517-522.
  15. Zang SQ., et al. “Comparison of Different Periodontal Healing of Critical Size Noncontained and Contained Intrabony Defects in Beagles”. Chinese Medical Journal 130.4 (2017): 477-486.
  16. Sculean A., et al. “Five-year results following treatment of intrabony defects with enamel matrix proteins and guided tissue regeneration”. Journal of Clinical Periodontology 31.7 (2004): 545-549.
  17. Sculean A., et al. “Ten-year results following treatment of intra-bony defects with enamel matrix proteins and guided tissue regeneration”. Journal of Clinical Periodontology 35.9 (2008): 817-824.
  18. Artzi Z., et al. “Deproteinized bovine bone in association with guided tissue regeneration or enamel matrix derivatives procedures in aggressive periodontitis patients: a 1-year retrospective study”. Journal of Clinical Periodontology 42.6 (2015): 547-556.
  19. Siciliano VI., et al. “Clinical outcomes after treatment of non-contained intrabony defects with enamel matrix derivative or guided tissue regeneration: a 12-month randomized controlled clinical trial”. Journal of Periodontology 82.1 (2011): 62-71.
  20. Zucchelli G., et al. “Enamel matrix proteins and guided tissue regeneration with titanium-reinforced expanded polytetrafluoroethylene membranes in the treatment of infrabony defects: a comparative controlled clinical trial”. Journal of Periodontology 73.1 (2002): 3-12.
  21. Regazzini PF., et al. “Comparative study of enamel matrix derivative with or without GTR in the treatment of class II furcation lesions in dogs”. The International Journal of Periodontics and Restorative Dentistry 24.5 (2004): 476-487.
  22. Jaiswal R and Deo V. “Evaluation of the effectiveness of enamel matrix derivative, bone grafts, and membrane in the treatment of mandibular Class II furcation defects”. The International Journal of Periodontics and Restorative Dentistry 33.2 (2013): e58-e64.
  23. Gamal AY., et al. “Crevicular Fluid Growth Factors Release Profile Following the Use of Platelet-Rich Fibrin and Plasma Rich Growth Factors in Treating Periodontal Intrabony Defects: A Randomized Clinical Trial”. Journal of Periodontology 87.6 (2016): 654-662.
  24. Chadwick JK., et al. “Clinical and Radiographic Evaluation of Demineralized Freeze-Dried Bone Allograft Versus Platelet-Rich Fibrin for the Treatment of Periodontal Intrabony Defects in Humans”. Journal of Periodontology 87.11 (2016): 1253-1260.

 

Citation: Nazli Rabienejad., et al. “Comparative Evaluation of Using Leukocyte-Platelet Rich Fibrin (L-PRF) and Emdogain (EMD) in Guided Tissue Regeneration (GTR) Technique for Reconstruction of Periodontal Intrabony Defects in Patients with Periodontitis”. EC Dental Science 19.2 (2020): 01-07.

PubMed Indexed Article


EC Pharmacology and Toxicology
LC-UV-MS and MS/MS Characterize Glutathione Reactivity with Different Isomers (2,2' and 2,4' vs. 4,4') of Methylene Diphenyl-Diisocyanate.

PMID: 31143884 [PubMed]

PMCID: PMC6536005


EC Pharmacology and Toxicology
Alzheimer's Pathogenesis, Metal-Mediated Redox Stress, and Potential Nanotheranostics.

PMID: 31565701 [PubMed]

PMCID: PMC6764777


EC Neurology
Differences in Rate of Cognitive Decline and Caregiver Burden between Alzheimer's Disease and Vascular Dementia: a Retrospective Study.

PMID: 27747317 [PubMed]

PMCID: PMC5065347


EC Pharmacology and Toxicology
Will Blockchain Technology Transform Healthcare and Biomedical Sciences?

PMID: 31460519 [PubMed]

PMCID: PMC6711478


EC Pharmacology and Toxicology
Is it a Prime Time for AI-powered Virtual Drug Screening?

PMID: 30215059 [PubMed]

PMCID: PMC6133253


EC Psychology and Psychiatry
Analysis of Evidence for the Combination of Pro-dopamine Regulator (KB220PAM) and Naltrexone to Prevent Opioid Use Disorder Relapse.

PMID: 30417173 [PubMed]

PMCID: PMC6226033


EC Anaesthesia
Arrest Under Anesthesia - What was the Culprit? A Case Report.

PMID: 30264037 [PubMed]

PMCID: PMC6155992


EC Orthopaedics
Distraction Implantation. A New Technique in Total Joint Arthroplasty and Direct Skeletal Attachment.

PMID: 30198026 [PubMed]

PMCID: PMC6124505


EC Pulmonology and Respiratory Medicine
Prevalence and factors associated with self-reported chronic obstructive pulmonary disease among adults aged 40-79: the National Health and Nutrition Examination Survey (NHANES) 2007-2012.

PMID: 30294723 [PubMed]

PMCID: PMC6169793


EC Dental Science
Important Dental Fiber-Reinforced Composite Molding Compound Breakthroughs

PMID: 29285526 [PubMed]

PMCID: PMC5743211


EC Microbiology
Prevalence of Intestinal Parasites Among HIV Infected and HIV Uninfected Patients Treated at the 1o De Maio Health Centre in Maputo, Mozambique

PMID: 29911204 [PubMed]

PMCID: PMC5999047


EC Microbiology
Macrophages and the Viral Dissemination Super Highway

PMID: 26949751 [PubMed]

PMCID: PMC4774560


EC Microbiology
The Microbiome, Antibiotics, and Health of the Pediatric Population.

PMID: 27390782 [PubMed]

PMCID: PMC4933318


EC Microbiology
Reactive Oxygen Species in HIV Infection

PMID: 28580453 [PubMed]

PMCID: PMC5450819


EC Microbiology
A Review of the CD4 T Cell Contribution to Lung Infection, Inflammation and Repair with a Focus on Wheeze and Asthma in the Pediatric Population

PMID: 26280024 [PubMed]

PMCID: PMC4533840


EC Neurology
Identifying Key Symptoms Differentiating Myalgic Encephalomyelitis and Chronic Fatigue Syndrome from Multiple Sclerosis

PMID: 28066845 [PubMed]

PMCID: PMC5214344


EC Pharmacology and Toxicology
Paradigm Shift is the Normal State of Pharmacology

PMID: 28936490 [PubMed]

PMCID: PMC5604476


EC Neurology
Examining those Meeting IOM Criteria Versus IOM Plus Fibromyalgia

PMID: 28713879 [PubMed]

PMCID: PMC5510658


EC Neurology
Unilateral Frontosphenoid Craniosynostosis: Case Report and a Review of the Literature

PMID: 28133641 [PubMed]

PMCID: PMC5267489


EC Ophthalmology
OCT-Angiography for Non-Invasive Monitoring of Neuronal and Vascular Structure in Mouse Retina: Implication for Characterization of Retinal Neurovascular Coupling

PMID: 29333536 [PubMed]

PMCID: PMC5766278


EC Neurology
Longer Duration of Downslope Treadmill Walking Induces Depression of H-Reflexes Measured during Standing and Walking.

PMID: 31032493 [PubMed]

PMCID: PMC6483108


EC Microbiology
Onchocerciasis in Mozambique: An Unknown Condition for Health Professionals.

PMID: 30957099 [PubMed]

PMCID: PMC6448571


EC Nutrition
Food Insecurity among Households with and without Podoconiosis in East and West Gojjam, Ethiopia.

PMID: 30101228 [PubMed]

PMCID: PMC6086333


EC Ophthalmology
REVIEW. +2 to +3 D. Reading Glasses to Prevent Myopia.

PMID: 31080964 [PubMed]

PMCID: PMC6508883


EC Gynaecology
Biomechanical Mapping of the Female Pelvic Floor: Uterine Prolapse Versus Normal Conditions.

PMID: 31093608 [PubMed]

PMCID: PMC6513001


EC Dental Science
Fiber-Reinforced Composites: A Breakthrough in Practical Clinical Applications with Advanced Wear Resistance for Dental Materials.

PMID: 31552397 [PubMed]

PMCID: PMC6758937


EC Microbiology
Neurocysticercosis in Child Bearing Women: An Overlooked Condition in Mozambique and a Potentially Missed Diagnosis in Women Presenting with Eclampsia.

PMID: 31681909 [PubMed]

PMCID: PMC6824723


EC Microbiology
Molecular Detection of Leptospira spp. in Rodents Trapped in the Mozambique Island City, Nampula Province, Mozambique.

PMID: 31681910 [PubMed]

PMCID: PMC6824726


EC Neurology
Endoplasmic Reticulum-Mitochondrial Cross-Talk in Neurodegenerative and Eye Diseases.

PMID: 31528859 [PubMed]

PMCID: PMC6746603


EC Psychology and Psychiatry
Can Chronic Consumption of Caffeine by Increasing D2/D3 Receptors Offer Benefit to Carriers of the DRD2 A1 Allele in Cocaine Abuse?

PMID: 31276119 [PubMed]

PMCID: PMC6604646


EC Anaesthesia
Real Time Locating Systems and sustainability of Perioperative Efficiency of Anesthesiologists.

PMID: 31406965 [PubMed]

PMCID: PMC6690616


EC Pharmacology and Toxicology
A Pilot STEM Curriculum Designed to Teach High School Students Concepts in Biochemical Engineering and Pharmacology.

PMID: 31517314 [PubMed]

PMCID: PMC6741290


EC Pharmacology and Toxicology
Toxic Mechanisms Underlying Motor Activity Changes Induced by a Mixture of Lead, Arsenic and Manganese.

PMID: 31633124 [PubMed]

PMCID: PMC6800226


EC Neurology
Research Volunteers' Attitudes Toward Chronic Fatigue Syndrome and Myalgic Encephalomyelitis.

PMID: 29662969 [PubMed]

PMCID: PMC5898812


EC Pharmacology and Toxicology
Hyperbaric Oxygen Therapy for Alzheimer's Disease.

PMID: 30215058 [PubMed]

PMCID: PMC6133268


News and Events


August Issue Release

We always feel pleasure to share our updates with you all. Here, notifying you that we have successfully released the August issue of respective journals and can be viewed in the current issue pages.

Submission Deadline for September Issue

Ecronicon delightfully welcomes all the authors around the globe for effective collaboration with an article submission for the September issue of respective journals. Submissions are accepted on/before August 21, 2020.

Certificate of Publication

Ecronicon honors with a "Publication Certificate" to the corresponding author by including the names of co-authors as a token of appreciation for publishing the work with our respective journals.

Best Article of the Issue

Editors of respective journals will always be very much interested in electing one Best Article after each issue release. The authors of the selected article will be honored with a "Best Article of the Issue" certificate.

Certifying for Review

Ecronicon certifies the Editors for their first review done towards the assigned article of the respective journals.

Latest Articles

The latest articles will be updated immediately on the articles in press page of the respective journals.

Immediate Assistance

The prime motto of this team is to clarify all the queries without any delay or hesitation to avoid the inconvenience. For immediate assistance on your queries please don't hesitate to drop an email to editor@ecronicon.uk