Research Article
Volume 9 Issue 4 - 2022
β2-Adrenoceptor and Expression of MuRF1 and Atrogin-1 Under Stress
Ricardo Moura, Marco Antonio Cordeiro, Alessandra Medeiros, Daniela Ortolani, Daniel Araki Ribeiro, Ana Elisa Teófilo Saturi de Carvalho and Regina Celia Spadari*

Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, SP, Brazil

*Corresponding Author: Regina Celia Spadari, Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, SP, Brazil.
Received: June 15, 2022; Published: June 18, 2022




Abstract

Background: Stress is an important risk factor for cardiovascular diseases. During the stress reaction, catecholamines released by the sympathetic nervous system and the adrenal medulla couple to β-adrenoceptors (β-AR) in almost every organ. In the heart, β1- (β1-AR) and β2-adrenoceptors (β2-AR) are expressed in an 80:20 ratio, and control heart functioning (via the Gs protein-adenylyl-cyclase signaling pathway) as well as cardiomyocytes life cycle (via the Gi-PI3K-Akt pathway). Changes in β1-AR/β2-AR ratio were associated with poor cardiac performance and can be triggered by stress. The putative consequences of those stress-induced changes on cardiac atrophy/pathological hypertrophy were not explored yet. This work aims to investigate the role played by β2-AR in the expression of components of the PI3K-Akt signaling pathway in the heart of rats submitted to foot shock stress.

Material and Method: Adult male rats were distributed in four groups: control, stress, ICI118,551 treated, and stress + ICI118,551 treated. Rats were submitted to one daily session of foot-shock stress (30 min duration, 1 mA, 1 s duration at random intervals between 5 and 25 s) during 3 days. ICI was administered i.p., 2 days before and during the stress period. The expression of proteins was analyzed by Western blot in the heart of rats sacrificed immediately after the last stress session.

Results: Foot-shock stress induced a reduction in the expression of MuRF1 whereas atrogin-1 and C2 proteasome expressions were unaltered. In the heart of stressed rats treated with the β2-AR antagonist (ICI118,551) there was down regulation of pAkt and atrogin-1 whereas MuRF1 and C2 proteasome were up regulated.

Conclusion: The β2-AR plays a role in the control of the signaling pathways leading to the expression of MuRF1, atrogin-1 and C2 proteasome under stress, probably protecting the heart against atrophy and pathological hypertrophy.

 

Keywords: β2 adrenoceptor; Atrogin-1; MuRF1; Cardiac Remodeling; Stress

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Citation: Regina Celia Spadari., et al. “β2-Adrenoceptor and Expression of MuRF1 and Atrogin-1 Under Stress”. EC Gynaecology 9.4 (2022): 20-30.

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