Research Article
Volume 7 Issue 3 - 2020
Simultaneous Measurement of Right Ventricular and Pulmonary Artery Pressures in a Rat to Hemodynamically Characterize Pulmonary Artery Hypertension
Filip Konecny*
Department of Surgery, Division of Plastic Surgery, McMaster University, Hamilton, ON, Canada
*Corresponding Author: Filip Konecny, Department of Surgery, Division of Plastic Surgery, McMaster University, Hamilton, ON, Canada.
Received: January 12, 2020; Published: February 12, 2020


Introduction: Pulmonary Artery (PA) hypertension is disease of pulmonary vasculature with final constrictive remodeling of pulmonary arterioles leading to (RV) dysfunction and right sided HF failure. Pre-clinical animal models have been developed to mimic this disease. In this methodology article, surgical access, ventilator set up and direct open chest measurements of PA hypertension are described in a rat model. As acute vascular access to the rat’s PA is complicated, mainly due to the anatomy of the right ventricle and the right ventricular outflow tract, to assure good hemodynamic data collection, open chest model was carefully designed using controlled mechanical volume ventilation (CMVV).

Methods and Aims: Setting of CMVV was adjusted to limit its influence on RV preload and LV afterload in the instance of chronic pulmonary disease. Volume-ventilation setting (tidal volume and respiration rate) was based on body weight. Isoflurane mono-anesthesia was used without any premedication. Chronic PA injury was induced by monocrotaline (MCT). PA pressures were compared using single and dual pressure catheter at 3-weeks post injury. Initially, single pressure catheter was positioned in the PA to assess data quality, while advanced data comparison (RV and PA pressures) during PA hypertension were made using dual pressure catheter. PA access was performed using “high” RV needle-stab, adjacent to the anatomical area of the PA outflow.

Results: Introduction of single pressure catheter was successful and collected data during RV systole and diastole did not produce any major pressure artefacts. Final position of the pressure catheter, in the main PA was guided by using visual cues i.e. distance of pressure sensor on the catheter, accompanied by simultaneous data recording from that location. In case of dual pressure catheter, insertion was also successful, and RV and PA pressure data were able to be recorded. In case of PA hypertension, systolic ranges were (41 - 52 mmHg) as compared to (25 - 30 mmHg), and diastolic (21 - 27 mmHg) vs. (9 - 14 mmHg); n = 4. Additionally, in case of hypertension, high afterload pressures complicated RV ejection, with PAP cresting about 1 mmHg higher than the maximal RVP, when overlaid. During further assessment, RV ejection was additionally complicated by higher PA dicrotic notch pressures, at the end of systole; for hypertension (37 - 41 mmHg) vs. naïve (16 - 21 mmHg), n = 4.

Keywords: Pulmonary Artery (PA) Hypertension; Open Chest Access; Acute Measurement; Solid-State Pressure Sensor; 1.6F Pressure Catheter


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Citation: Filip Konecny. “Simultaneous Measurement of Right Ventricular and Pulmonary Artery Pressures in a Rat to Hemodynamically Characterize Pulmonary Artery Hypertension”. EC Cardiology 7.3 (2020): 01-10.

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