Research Article
Volume 3 Issue 1 - 2020
Validation of a LC-MS Assay Applied to Synovial Fluid for Detection for Periprosthetic Joint Infection
Riccardo Dall’Anese1*, Giuseppe Bartolini1, Fiamma Balboni1, Massimo Quercioli2, Giovanni Balato3, Paola Pezzati2 and Andrea Baldini4
1Istituto Fiorentino Cura ed Assistenza Firenze (iFCA), Laboratorio Analisi Chimico-Cliniche, Via Del Pergolino, Florence, Italy
2Centro Regionale di Riferimento SOD Sicurezza e Qualità. Azienda Ospedaliero Universitaria Careggi, Via delle Oblate, Florence, Italy
3Università Federico II di Napoli, Scuola di Medicina e Chirurgia, Dipartimento di Sanità Pubblica, Via Pansini, Naples, Italy
4Istituto Fiorentino Cura ed Assistenza Firenze (iFCA), UO Ortopedia, Via Del Pergolino, Florence, Italy
*Corresponding Author:Riccardo Dall’Anese, Istituto Fiorentino Cura ed Assistenza Firenze (iFCA), Laboratorio Analisi Chimico-Cliniche, Via Del Pergolino, Florence, Italy.
Received: November 25, 2019; Published: December 18, 2019


The presence of Alpha defensin in synovial fluids are a potential biomarker for identification of periprosthetic infections: the identification method are based on proteomic test after enzymatic digestion of synovial fluid. The method can be used as screening method on all patients undergoing prosthetic review to distinguish cases of inflammation from infection with alternative methods (clinical or ELISA test). The preliminary step of research it’s the check of uniqueness of possible peptide derived from trypsin digestion of alpha denesin. This step of work was performed on real positive sample (compared with negative sample to confirm): after a sample preparation, the detection of peptide was carried out by liquid chromatography - time of flight mass spectrometry (LC-QTOF). The obtained data have been developed by proteomic software to identify the uniqueness biomarker peptide for alpha defensin and ensure that aren’t any other human proteins able to generate identical or similar peptide after tryptic digestion. The analytical method was subsequently validated according to the CLSI and FDA guidelines in order to verify the method specifications in accordance with the requirements for the application of the method in diagnostic protocols.

Keywords: Synovial Fluid (SF); LC-MS Assay; Periprosthetic Joint Infection


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Citation: Riccardo Dall’Anese., et al. “Validation of a LC-MS Assay Applied to Synovial Fluid for Detection for Periprosthetic Joint Infection”. EC Clinical and Medical Case Reports 3.1 (2020): 01-14.

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