Research Article
Volume 3 Issue 11 - 2020
Rapid Genetic Diversity with Variability between Replicated Digital Organism Simulations and its Implications on Cambrian Explosion
Shermaine SM Chew1,2, Madhurya V Murthy1,2, Nur Jannah Kamarudin1,2, Victor CC Wang1,2, Xue Ting Tan1,2, Avettra Ramesh1,2, Nikita V Yablochkin1,2, Karthiga Mathivanan1,2 and Maurice HT Ling1,2,3*
1Department of Applied Sciences, Northumbria University, United Kingdom
2School of Life Sciences, Management Development Institute of Singapore, Singapore
3HOHY PTE LTD, Singapore
*Corresponding Author: Maurice HT Ling, School of Life Sciences, Management Development Institute of Singapore, Singapore.
Received: October 05, 2020; Published: October 31, 2020


Cambrian Explosion resulted in substantial increase in biodiversity, which may be attributed to both environmental and biological factors. Although increased genetic evolution rate had been shown during this period, the role of genetic evolution in increased biodiversity is unclear. Re-creating Cambrian Explosion experimentally is not feasible. In this study, we used digital organisms (DOs) at high rate of random point mutations in the absence of selective pressure to examine the extent genetic evolution possible during Cambrian Explosion. Our simulation results suggest rapid and significant genetic divergence in the absence of selective pressure can occur at a species level and at local population level with significant differences between each local population (F ≥ 15.97, p-value ≤ 1.4E-79). Hence, the emergence of biodiversity in Cambrian Explosion may be due to the release of accumulated adaptive potential.

Keywords: Cambrian Explosion; Biodiversity; Digital Organisms (DOs)


  1. Holland PWH. “Did Homeobox Gene Duplications Contribute to the Cambrian explosion?” Zoological Letters 1 (2015): 1.
  2. Fox D. “What Sparked the Cambrian Explosion?” Nature7590 (2016): 268-270.
  3. Smith MP and Harper DAT. “Causes of the Cambrian Explosion”. Science6152 (2013): 1355-1356.
  4. Hughes NC and Heim NA. “PALAEOZOIC | Cambrian”. In: Encyclopedia of Geology. Elsevier (2005): 163-175.
  5. Zhang X and Shu D. “Causes and Consequences of the Cambrian Explosion”. Science in China Series D-Earth Sciences 5 (2014): 930-942.
  6. Sperling EA., et al. “Oxygen, Ecology, and the Cambrian Radiation of Animals”. Proceedings of the National Academy of Sciences of the United States of America 33 (2013): 13446-13451.
  7. Wood R., et al. “Integrated Records of Environmental Change and Evolution Challenge the Cambrian Explosion”. Nature Ecology and Evolution 4 (2019): 528-538.
  8. Erwin DH., et al. “The Cambrian Conundrum: Early Divergence and Later Ecological Success in the Early History of Animals”. Science6059 (2011): 1091-1097.
  9. Lee MSY., et al. “Rates of Phenotypic and Genomic Evolution During the Cambrian explosion”. Current Biology 19 (2013): 1889-1895.
  10. Langton CG. “Studying Artificial Life with Cellular Automata”. Physica D: Nonlinear Phenomena 1-3 (1986): 120-149.
  11. Elena SF and Sanjuan R. “The Effect of Genetic Robustness on Evolvability in Digital Organisms”. BMC Evolutionary Biology 8 (2008): 284.
  12. Anderson CJR and Harmon L. “Ecological and Mutation-Order Speciation in Digital Organisms”. The American Naturalist 2 (2014): 257-268.
  13. Wilke CO., et al. “Evolution of Digital Organisms at High Mutation Rates Leads to Survival of the Flattest”. Nature6844 (2001): 331-333.
  14. Castillo CFG, Ling MHT. "Resistant Traits in Digital Organisms Do Not Revert Preselection Status Despite Extended Deselection: Implications to Microbial Antibiotics Resistance." BioMed Research International 2014 (2014): 648389.
  15. Ling MH. “Applications of Artificial Life and Digital Organisms in the Study of Genetic Evolution”. Advances in Computer Science: An International Journal 4 (2014): 107-112.
  16. Yao Y., et al. “Using Digital Organisms to Study the Evolutionary Consequences of Whole Genome Duplication and Polyploidy.”. PloS One7 (2019): e0220257.
  17. Castillo CF., et al. “Resistance Maintained in Digital Organisms Despite Guanine/Cytosine-Based Fitness Cost and Extended De-Selection: Implications to Microbial Antibiotics Resistance”. MOJ Proteomics & Bioinformatics2 (2015): 00039.
  18. Wilke CO and Adami C. “The Biology of Digital Organisms”. Trends in Ecology and Evolution 11 (2002): 528-532.
  19. C Castillo CF and Ling MH. “Digital Organism Simulation Environment (DOSE): A Library for Ecologically-Based In Silico Experimental Evolution”. Advances in Computer Science: An International Journal1 (2014): 44-50.
  20. Castillo CF and Ling MH. “Digital Organism Simulation Environment (DOSE) Version 1.0.4”. In: Current STEM, Volume 1. Nova Science Publishers, Inc (2018): 1-106.
  21. Rattray AJ and Strathern JN. “Error-Prone DNA Polymerases: When Making a Mistake is the Only Way to Get Ahead”. Annual Review of Genetics 37 (2003): 31-66.
  22. Lee DF., et al. “Mapping DNA Polymerase Errors by Single-Molecule Sequencing”. Nucleic Acids Research 13 (2016): e118.
  23. Kwek BZ., et al. “Random Sequences May Have Putative Beta-Lactamase Properties”. Acta Scientific Medical Sciences 7 (2019): 113-117.
  24. Ardhanari-Shanmugam KD., et al. “De Novo Origination of Bacillus subtilis 168 Promoters from Random Sequences”. Acta Scientific Microbiology 11 (2019): 07-10.
  25. Usman S., et al. “Pseudomonas balearica DSM 6083T Promoters Can Potentially Originate from Random Sequences”. MOJ Proteomics & Bioinformatics 2 (2019): 66-70.
  26. Smith TF and Waterman MS. “Identification of Common Molecular Subsequences”. Journal of Molecular Biology 1 (1981): 195-197.
  27. Ling MHT. “Seq Properties: A Python Command-Line Tool for Basic Sequence Analysis”. Acta Scientific Microbiology 6 (2020): 103-106.
  28. Bardapurkar AS. “What is “Natural” in Natural Selection?” Resonance5 (2013): 475-482.
  29. O’Donnell DR., et al. “The Roles of Standing Genetic Variation and Evolutionary History in Determining the Evolvability of Anti-Predator Strategies”. PloS One6 (2014): e100163.
  30. Lai Y-T., et al. “Standing Genetic Variation as the Predominant Source for Adaptation of a Songbird”. Proceedings of the National Academy of Sciences 6 (2019): 2152-2157.
  31. Blount ZD., et al. “Historical Contingency and the Evolution of a Key Innovation in an Experimental Population of Escherichia coli”. Proceedings of the National Academy of Sciences of the United States of America 23 (2008): 7899-7906.
  32. Leon D., et al. “Innovation in an coli Evolution Experiment is Contingent on Maintaining Adaptive Potential Until Competition Subsides”. PLoS Genetics 14.4 (2018): e1007348.
Citation: Shermaine SM Chew., et al. “Rapid Genetic Diversity with Variability between Replicated Digital Organism Simulations and its Implications on Cambrian Explosion”. EC Clinical and Medical Case Reports 3.11 (2020): 64-68.

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