Research Article
Volume 3 Issue 7 - 2020
Concept for Possible Development of Vaccine against Corona in Escherichia coli K-12, Yale C600 Strain
Nitosh Kumar Brahma*
Convener Chemical Engineering, Div, Wbsc/Iei, Visiting Professor, Institute of Genetic Engineering, Badu, Kolkata, India
*Corresponding Author: Nitosh Kumar Brahma, Convener Chemical Engineering, Div, Wbsc/Iei, Visiting Professor, Institute of Genetic Engineering, Badu, Kolkata, India.
Received: April 25, 2020; Published: June 29, 2020




Abstract

An AAIR (Anti-adherent Immune Response) as vaccine against n-COVID-19 could possibly be developed in support of Genetically Engineered (GE) Escherichia coli k-12, C600 Yale strain, if cloned with any of spike protein of infected n-COVID-19 virus and ACE-I and II receptor protein genes of lung cells. The developed antibody will suppress/block ACE- I and II (Angiotensin Converting Enzyme) of the lung cells. Antibody development against infected spike protein of n-COVID-19 would be difficult, since the n-COVID-19 viruses are changing their character rapidly. The receptor proteins of lung cells are more stable immunologically to develop antibodies. These antibodies will remain active to block the receptor of the lung cells, as soon as these antibodies will recognise that virus n-COVID-19 is entered body and is attempting to attack by changing body metabolic system. To develop hybrid genetically engineered (GE) Escherichia coli k-12 C600 Yale strain, the personal knowledge of the author with 026:EPEC (Enteropathogenic Escherichia coli) and the antibody development with GE bacteria has been highlighted. An AAIR against 026:EPEC fatal diarrhoea was possible to develop, when a GE hybrid E. coli k-12, MRHU (+) was possible to design and when MRHU (+) 95 MD, plasmid was possible to transfer into E. coli k-12 C600 Nalidixic acid (nal) and Streptomycin (sm) resistant recipient (F-) Yale strain by Co-conjugation. At (F+ x F+ pRT x F-) conjugations and at vigorous selections, in vivo on selective agars. A GE Hybrid E. coli k-12, 5405, GE E. coli k-12 at 10-3 frequency was possible to isolate and was used in AAIR on Balb/c mice. Mice were protected against fatal diarrhoea of 026:EPEC when the donors (wild types) were challenged on same Balb/c mice. The results were reviewed, and the insight of those findings are being used in the present AAIR model to develop of n-COVID-19 vaccine, as ultimate solution to protect mankind. Since SARS-COV-2, virus and n-COVID-19 infection possess very unknown nature of spreading. As per the author AAIR for n-COVIC-19 would be very complicated, complex, the combined model of coccus/bacteroid and retrovirus even in their adhering and propagating natures on the surface and the inside of the lung cells. The experimental knowledge derived from 026: EPEC AAIR on Balb/c are therefore been partially used in this concept.

Keywords: Vaccine; Corona Virus; Escherichia coli K-12

References

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  2. Brahma NK. “Genetically Engineered Hybrid 5405 E. coli K-12 fimbriae in vaccination With Balb/c mice against 026:serotype EPEC diarrhea”. Indian Journal of Chemical Engineers (ICE) 1 (1999): 45-52.
  3. Brahma NK. “Cell and Tissues in Biomedical Applications”. Trends in Biomaterials and Artificial Organs 2 (2003): 17-23.
  4. Brahma NK. “MRHA and sero-precipitations with 08: ETEC & 026: EPEC surface antigens”. Indian Journal of Microbiology 3 (1993): 2229.
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  6. Brahma NK. “Hybrid blended Polymer of bacterial surface protein (fimbriae= pili= BNT (Bionanotube) as an important Vaccinating and drug delivery Tools: A Biomedical design”. Trends in Biomaterials and Artificial Organs (2007).
  7. Gupta R., et al. “Application of Plasmid Image processing for Rapid Diagnosis in case of Microbial infections”. International Conference of IEEE on Image analysis (2007).
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  10. Braude AI., et al. “Medical Microbiology and Infectious Diseases”. Saunders Publication, UK (1986).
Citation: Nitosh Kumar Brahma. “Concept for Possible Development of Vaccine against Corona in Escherichia coli K-12, Yale C600 Strain”. EC Clinical and Medical Case Reports 3.7 (2020): 111-116.

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