Review Article
Volume 3 Issue 5 - 2020
Current Insights into the Genomics of Novel Coronavirus: SARS-Cov-2
Rabia Farooq1*, Sabhiya Majid2 and Aamir Hanif3
1Assistant Professor, Department of Biochemistry, College of Medicine, University of Bisha, Saudi Arabia
2Professor and Head, Department of Biochemistry, Government Medical College, Srinagar, India
3Department of Chemistry, Faculty of Sciences, Bisha University, Saudi Arabia
4Registrar Academics, Government Medical College, Srinagar, India
*Corresponding Author: Rabia Farooq, Assistant Professor, Department of Biochemistry, College of Medicine, University of Bisha, Saudi Arabia.
Received: March 17, 2020; Published: April 07, 2020




Abstract

Coronavirus (2019 nCoV)- a deadly virus which causes panic throughout the world particularly in Wuhan region of China in 2020 affecting more than 12000 people as on 02-02-2020. Coronaviruses belong to a class of single stranded RNA viruses from the order Nidovirales. The word “Corona” itself means crown taken from its structure due to its large bulb like outgrowths, known to be among largest RNA virus, size ranging from 27 to 32 Kb. This virus is known to have unique strategy for replication. This review will lay emphasis on various animal coronaviruses and their pathogenesis, and the newly discovered human emerging pathogen 2019 - nCoV, no treatment i.e. no vaccines or antiviral drugs are approved for its prevention or treatment. This review will help understand the biology and potential risk of coronaviruses.

Keywords: Coronavirus; Wuhan; Vaccine; 2019-nCoV

References

  1. Malcolm R Macnaughton. “Occurrence and Frequency of Coronavirus Infections in Humans as Determined by Enzyme-Linked Immunosorbent Assay”. Infection and Immunity (1982): 419-423.
  2. Bradburne AF and DAJ Tyrrell. “Coronaviruses of man”. Progress in Medical Virology 13 (1971): 373-403.
  3. Pyrc K., et al. “Identification of new human coronaviruses”. Expert Review of Anti-infective Therapy 5 (2007): 245-253.
  4. Zhong N. “Management and prevention of SARS in China”. Philosophical Transactions of the Royal Society B: Biological Sciences 359 (2004): 1115-1116.
  5. Peiris JS., et al. “Severe acute respiratory syndrome”. Nature Medicine 10 (2004): S88-S97.
  6. Lu L., et al. “Middle East respiratory syndrome coronavirus (MERS-CoV): challenges in identifying its source and controlling its spread”. Microbes and Infection 15 (2013): 625-629.
  7. Lau SK., et al. “Discovery of a novel coronavirus, China Rattus coronavirus HKU24, from Norway rats supports the murine origin of Betacoronavirus 1 and has implications for the ancestor of Betacoronavirus lineage A”. Journal of Virology 89 (2015): 3076-3092.
  8. Anthony SJ., et al. “Coronaviruses in bats from Mexico”. Journal of General Virology 94 (2013): 1028-1038.
  9. Peiris JS., et al. “Coronavirus as a possible cause of severe acute respiratory syndrome”. Lancet 361 (2003): 1319-1325.
  10. Zaki AM., et al. “Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia”. The New England Journal of Medicine 367 (2012): 1814-1820.
  11. Van Boheemen S., et al. “Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans”. MBio (2012): 312.
  12. Wang N., et al. “Structure of MERS-CoV spike receptor-binding domain complexed with human receptor DPP4”. Cell Research 23 (2013): 986-993.
  13. Guery B., et al. “Clinical features and viral diagnosis of two cases of infection with Middle East Respiratory Syndrome coronavirus: a report of nosocomial transmission”. Lancet 20381 (2013): 2265-2272.
  14. Reusken CB., et al. “Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study”. The Lancet Infectious Diseases (2013).
  15. Fox Dan. "What you need to know about the Wuhan coronavirus". Nature (2020).
  16. Roujian Lu., et al. “Genomic characterisation and epidemiology of 2019 novel coronavirus : implications for virus origins and receptor binding”. Lancet (2019).
  17. Severe acute respiratory syndrome coronavirus 2 (2019).
  18. "Wuhan seafood market pneumonia virus isolate Wuhan-Hu-1, complete genome". National Center for Biotechnology Information. United States: National Institutes of Health (2020).
  19. Zhu Na., et al. “A Novel Coronavirus from Patients with Pneumonia in China, 2019". New England Journal of Medicine8 (2020): 727-733.
  20. Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome.
  21. ORF10 protein [Severe acute respiratory syndrome coronavirus 2].
  22. Clover Initiates Development of Recombinant Subunit-Trimer Vaccine for Wuhan Coronavirus (2019-nCoV).
  23. Masters PS. “The molecular biology of coronaviruses”. Advances in Virus Research 66 (2006): 193-292.
  24. Wang C., et al. “MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular immunity in rhesus macaques”. Oncotarget8 (2017): 12686-12694.
  25. DeDiego ML., et al. “A severe acute respiratory syndrome coronavirus that lacks the E gene is attenuated In vitro and In vivo”. Journal of Virology 4 (2007): 1701-1713.
  26. Ruch TR and Machamer CE. “The coronavirus E protein: Assembly and beyond”. Viruses3 (2012): 363-382.
  27. Kirchdoerfer RN., et al. “Pre-fusion structure of a human coronavirus spike protein”. Nature7592 (2016): 118-121.
  28. Song HC., et al. “Synthesis and characterization of a native, oligomeric form of recombinant severe acute respiratory syndrome coronavirus spike glycoprotein”. Journal of Virology 19 (2004): 10328-10335.
  29. McBride R., et al. “The coronavirus nucleocapsid is a multifunctional protein”. Viruses8 (2014): 2991-3018.
  30. Ruch TR and Machamer CE. “The hydrophobic domain of infectious bronchitis virus E protein alters the host secretory pathway and is important for release of infectious virus”. Journal of Virology2 (2011): 675-685.
  31. Masters PS. “The molecular biology of coronaviruses”. Advances in Virus Research 66 (2006): 193-292.
  32. Neuman BW., et al. “A structural analysis of M protein in coronavirus assembly and morphology”. Journal of Structural Biology 1 (2011): 11-22.
  33. De Haan CA., et al. “Assembly of the coronavirus envelope: homotypic interactions between the M proteins”. Journal of Virology 11 (2000): 4967-4978.
  34. Lim K and Liu D. “The missing link in coronavirus assembly: retention of the avian coronavirus infectious bronchitis virus envelope protein in the pre-Golgi compartments and physical interaction between the envelope and membrane proteins”. Journal of Biological Chemistry 20 (2001): 17515-17523.
  35. Mortola E and Roy P. “Efficient assembly and release of SARS coronavirus-like particles by a heterologous expression system”. FEBS Letters 1-2 (2004): 174-178.
  36. Fehr AR and Perlman S. “Coronaviruses: An overview of their replication and pathogenesis”. Coronaviruses: Springer (2015): 1-23.
  37. Escors D., et al. “The membrane M protein carboxy terminus binds to transmissible gastroenteritis coronavirus core and contributes to core stability”. Journal of Virology 3 (2001): 1312-1324.
  38. Narayanan K., et al. “Characterization of the coronavirus M protein and nucleocapsid interaction in infected cells”. Journal of Virology 17 (2000): 8127-8134.
  39. Venkatagopalan P., et al. “Coronavirus envelope (E) protein remains at the site of assembly”. Virology 478 (2015): 75-85.
  40. Nieto-Torres JL., et al. “Subcellular location and topology of severe acute respiratory syndrome coronavirus envelope protein”. Virology 2 (2011): 69-82.
  41. Ortego J., et al. “Generation of a replication-competent, propagation-deficient virus vector based on the transmissible gastroenteritis coronavirus genome”. Journal of Virology 22 (2002): 11518-11529.
  42. Münz M., et al. “The role of flexibility and conformational selection in the binding promiscuity of PDZ domains”. PLOS Computational Biology 11 (2012): e1002749.
  43. Javier RT and Rice AP. “Emerging theme: cellular PDZ proteins as common targets of pathogenic viruses”. Journal of Virology 22 (2011): 11544-11556.
  44. Jimenez-Guardeño JM., et al. “Identification of the mechanisms causing reversion to virulence in an attenuated SARS-CoV for the design of a genetically stable vaccine”. PLOS Pathogens 10 (2015): e1005215.
  45. Wu K., et al. “Crystal structure of NL63 respiratory coronavirus receptor-binding complexed with its human receptor”. Proceedings of the National Academy of Sciences of the United States of America 106 (2009): 19970-19974.
  46. Lu G., et al. “Molecular basis of binding between novel human coronavirus MERS-CoV and its receptor CD26”. Nature 500 (2013): 227-231.
  47. Chen YQ., et al. “Crystal structure of the receptor-binding domain from newly emerged Middle East respiratory syndrome coronavirus”. Journal of Virology 87 (2013): 10777-10783.
  48. Michael Letko and Vincent Munster. “Functional assessment of cell entry and receptor usage for lineage B β-coronaviruses, including 2019-nCoV” (2020).
  49. Towler P., et al. “ACE2 X-ray structures reveal a large hinge-bending motion important for inhibitor binding and catalysis”. Journal of Biological Chemistry 279 (2004): 17996-18007.
  50. Keidar S., et al. “ACE2 of the heart: from angiotensin I to angiotensin (1-7) ”. Cardiovascular Research 73 (2007): 463-469.
  51. Boehm M and Nabel EG. “Angiotensin-converting enzyme 2–a new cardiac regulator”. The New England Journal of Medicine 347 (2002): 1795-1797.
  52. Schwegmann-Wessels C and Herrler G. “Sialic acids as receptor determinants for coronaviruses”. Glycoconjugate Journal 23 (2006): 51-58.
  53. Dove A. “The bittersweet promise of glycobiology”. Nature Biotechnology 19 (2001): 913-917.
  54. Ghazarian H., et al. “A glycobiology review: carbohydrates, lectins and implications in cancer therapeutics”. Acta Histochemica 113 (2011): 236-247.
  55. Holmes KV., et al. “Virus-receptor interactions in the enteric tract. Virus-receptor interactions”. Advances in Experimental Medicine and Biology 412 (1997): 125-133.
  56. Bosch BJ., et al. “The coronavirus spike protein is a class I virus fusion protein: structural and functional characterization of the fusion core complex”. Journal of Virology 77 (2003): 8801-8811.
  57. Chu VC., et al. “Avian infectious bronchitis virus enters cells via the endocytic pathway”. Advances in Experimental Medicine and Biology 581 (2006): 309-312.
  58. Eifart P., et al. “Role of endocytosis and low pH in murine hepatitis virus strain A59 cell entry”. Journal of Virology (2007).
  59. Sawicki SG., et al. “A contemporary view of coronavirus transcription”. Journal of Virology 81 (2007): 20-29.
  60. Masters PS. “The molecular biology of coronaviruses”. Advances in Virus Research 66 (2006): 193-292.
  61. Susan RW and Sonia N. “Coronavirus pathogenesis and the emerging pathogen severe acute respiratory syndrome coronavirus”. Microbiology and Molecular Biology Reviews 69 (2005): 635-664.
  62. Namy O., et al. “A mechanical explanation of RNA pseudoknot function in programmed ribosomal frameshifting”. Nature 441 (2006): 244-247.
  63. Thiel V., et al. “Mechanisms and enzymes involved in SARS coronavirus genome expression”. Journal of General Virology 84 (2003): 2305-2315.
  64. Denison MR., et al. “Intracellular processing of the N-terminal ORF 1a proteins of the coronavirus MHV-A59 requires multiple proteolytic events”. Virology 189 (1992): 274-284.
  65. Slot JW and HJ Geuze. “Cryosectioning and immunolabeling”. Nature Protocols 2 (2007): 2480-2491.
  66. Ziebuhr J., et al. “Virus-encoded proteinases and proteolytic processing in the Nidovirales”. Journal of General Virology 81 (2000): 853-879.
  67. Ivanov KA., et al. “Major genetic marker of nidoviruses encodes a replicative endoribonuclease”. Proceedings of the National Academy of Sciences of the United States of America 101 (2004): 12694-12699.
  68. Kang H., et al. “Biochemical and genetic analyses of murine hepatitis virus Nsp15 endoribonuclease”. Journal of Virology 81 (2007): 13587-13597.
  69. Minskaia E., et al. “Discovery of an RNA virus 3′→5′ exoribonuclease that is critically involved in coronavirus RNA synthesis”. Proceedings of the National Academy of Sciences of the United States of America (2006).
  70. Seybert A., et al. “A complex zinc finger controls the enzymatic activities of nidovirus helicases”. Journal of Virology 79 (2005): 696-704.
  71. Bi W., et al. “Localization of mouse hepatitis virus open reading frame 1A derived proteins”. Journal for Neurovirology 4 (1998): 594-605.
  72. Gosert R., et al. “RNA replication of mouse hepatitis virus takes place at double-membrane vesicles”. Journal of Virology 76 (2002): 3697-3708.
  73. Snijder EJ., et al. “Ultrastructure and origin of membrane vesicles associated with the severe acute respiratory syndrome coronavirus replication complex”. Journal of Virology 80 (2006): 5927-5940.
  74. Knoops K., et al. “SARS-coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum”. PLOS Biology 6 (2008): e226.
  75. Oostra M., et al. “Localization and membrane topology of coronavirus nonstructural protein 4: involvement of the early secretory pathway in replication”. Journal of Virology 81 (2007): 12323-12336.
  76. Verheije MH., et al. “Mouse hepatitis coronavirus RNA replication depends on GBF1-mediated ARF1 activation”. PLOS Pathogens 4 (2008): e1000088.
Citation: Rabia Farooq., et al. “Current Insights into the Genomics of Novel Coronavirus: SARS-Cov-2”. EC Clinical and Medical Case Reports 3.5 (2020): 03-11.

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