Research Article
Volume 3 Issue 2 - 2020
Essential Oil of Cultivated Satureja khuzestanica Jamzad Improves Activities and Genes Expression of Hepatoglycoregulatory Enzymes in Diabetic Rats
Gholamreza Shahsavari1,2, Hassan Ahmadvand1,2 and Mobin Khoshbin1*
1Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
2Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
*Corresponding Author: Mobin Khoshbin, Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Received: November 12, 2019; Published: January 23, 2020




Abstract

Objective: The aim of the present study is to evaluate the effect of cultivated Satureja khuzestanica essential oil (SKEO) on activity and gene expression of hepatic glucokinase (GK), glycogen phosphorylase (GP) and phosphoenolpyruvate carboxykinase (PEPCK) in normal and diabetic rats. 

Methods: Thirty two wistar male rats were divided into four groups randomly; group one as control, group two as diabetic untreated, group three as sham treated with cultivated SKEO (100 mg/kg/d) in aqueous solution orally for 21 days, and group four as diabetic treated with cultivated SKEO (100 mg/kg/d) in aqueous solution orally for 21 days. After 21 days, animals were anaesthetized, liver were then removed immediately and used fresh or kept frozen until analysis of GK, GP and PEPCK activity and gene expression of GK, GP and PEPCK by using the quantitative real-time RT-PCR technique. 

Results: Hepatic GK activity and gene expression of GK in diabetic treated groups compared with diabetic untreated groups were significantly increased. Hepatic GP and PEPCK activity and gene expression of GP and PEPCK in diabetic treated groups compared with diabetic untreated groups were significantly decreased. Also, hepatic activity of GK, GP and PEPCK activity correlated positively with their gene expression. 

Conclusion: This study showed that SKEO might be a exert beneficial effects on activities of GK, GP and PEPCK and gene expression of GK, GP and PEPCK in diabetic rats. Therefore, SKEO may contribute to reduction of serum glucose, which seems to be related to its antioxidant properties.

Keywords: Antihyperglycemic; Gene Expression; Glucokinase; Glycogen Phosphorylase; Phosphoenolpyruvate Carboxykinase; Satureja khuzestanica Essential Oil

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Citation: Mobin Khoshbin., et al. “Essential Oil of Cultivated Satureja khuzestanica Jamzad Improves Activities and Genes Expression of Hepatoglycoregulatory Enzymes in Diabetic Rats”. EC Clinical and Medical Case Reports 3.2 (2020): 01-13.

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