Research Article
Volume 3 Issue 2 - 2020
"Spleen Organometry of Old Male Albino Rats After Whole-Body Exposure to Toluene"
Vladimir Nikolaevich Voloshin1*, Irina Sergeevna Voloshina2, Dmitry Alekseevich Lugovskov3, Alexander Nikolaevich Nesterenko3, Ida Yakovlevna Kozhemyaka1 and Maxim Gennadievich Grischuk4
1Department of Human Anatomy, St. Luke Lugansk State Medical University, Ukraine
2Department of Radiology, St. Luke Lugansk State Medical University, Ukraine
3Department of Stomatology, St. Luke Lugansk State Medical University, Ukraine
4Department of Pathological Physiology, St. Luke Lugansk State Medical University, Ukraine
*Corresponding Author:Vladimir Nikolaevich Voloshin, Department of Human Anatomy, St. Luke Lugansk State Medical University, Ukraine.
Received: November 20, 2019; Published: January 07, 2020


Spleen is a secondary lymphoid organ that is highly sensitive to different chemicals. Toluene is an aromatic hydrocarbon commonly used as an industrial solvent and can be considered as a potential immunotoxin. We aimed to investigate the organometry of the spleen from rats exposed to toluene. The toxicity of toluene was evaluated in male albino rats via whole-body exposure. The animals were exposed to target concentrations of 0 (air control) and 133 ppm of toluene in air for 5 hours/day, 5 days/week, for 2 month. The animals were weighted and decapitated at different time points (one, seven, sixteen, thirty one and sixty one day) post-exposure. The weight, length, width and thickness of the spleen were measured. It was studied absolute and relative weight of the spleen.

Rats that were withdrawn from the experiment 30 days after the cessation of toluene had an organ weight of 1134.67 mg. This indicator was not statistically significantly higher than the data of the respective control group by 5.75% (p = 0.109). The weight of the organ of rats of group 5 of series T was at the level of 1217.33 mg, which was 103.11% (p = 0.222) to the control. Rats that were withdrawn from the experiment 7 and 15 an organ length of 41.64 mm and 43.04 mm, respectively. In the 4 and 5 groups of rats, the average length of the organ was 104.71% (p = 0.076) and 97.82% (p = 0.282) to the data of the corresponding groups of the control series. Rats withdrawn from the experiment 15 and 30 days after toluene cessation had an organ thickness of 4.91 mm and 4.97 mm, which was 100.40% (p = 0.729) and 103.11% (p = 0.927) to the control indicators. The results demonstrate that inhaled toluene does not significantly affect the weight and size of the spleen of old rats.

Keywords: Toluene; Toxicity; Rat; Spleen


  1. Iwasaki A and R Medzhitov. “Regulation of adaptive immunity by the innate immune system”. Science 327 (2010): 291-295.
  2. Rey A., et al. “When immune-neuroendocrine interactions are disrupted: experimentally induced arthritis as an example”. Neuroimmunomodulation 17 (2010): 165-168.
  3. Tometten M., et al. “Nerve growth factor in reproductive biology: link between the immune, endocrine and nervous system?” Chemical Immunology and Allergy 89 (2005): 135-148.
  4. Hemdan NYA., et al. “The role of T helper (TH) 17 cells as a double-edged sword in the interplay of infection and autoimmunity with a focus on xenobiotic-induced immunomodulation”. Clinical and Developmental Immunology (2013): 374769.
  5. Michaud M., et al. “Autophagy dependent anticancer immune responses induced by chemotherapeutic agents in mice”. Science 334 (2011): 1573-1577.
  6. Vandenberg LN., et al. “Hormones and endocrine-disrupting chemicals: low-dose effects and nonmonotonic dose responses”. Endocrine Reviews 33 (2012): 378-345.
  7. Gopinath M and R Dhanasekar. “Microbial degradation of toluene”. African Journal of Biotechnology 11 (2012): 16210-16219.
  8. Angel MJ and GB Young. “Metabolic encephalopathies”. Neurologic Clinics 29 (2011): 837-882.
  9. Hanley KW., et al. “Exposure to o-toluidine, aniline, and nitrobenzene in a rubber chemical manufacturing plant: A retrospective exposure assessment update”. Journal of Occupational and Environmental Hygiene 8 (2012): 478-490.
  10. Rossi F and A. Nicolini. “Investigation on thermophysical properties of thymic cell cultures exposed to electromagnetic fields”. Environment and Pollution 2 (2013): 11-18.
  11. Poon R., et al. “Inhalation toxicity study of methanol, toluene, and methanol/toluene mixtures in rats: effects of 28-day exposure”. Toxicology and Industrial Health 10 (1994): 231-245.
  12. Ono A., et al. “Reproductive and developmental toxicity studies of toluene. II. Effects of inhalation exposure on fertility in rats”. Journal of Environmental Pathology, Toxicology and Oncology 1 (1996): 9-20.
  13. Nain S., et al. “Immunotoxicity and disease resistance in Japanese quail (Corturnixcoturnix japonica) exposed to malathion”. Ecotoxicology 20 (2011): 892-900.
  14. Tomlinson MJ. “Tissue engineering the thymus and secondary lymphoid organs”. Journal of Postdoctoral Research 1 (2013): 12-27.
  15. Haley P., et al. “STP position paper: best practices guideline for the routine pathology evaluation of the immune system”. Toxicology Pathology 33 (2005): 404-407.
  16. Maronpot RR. “A monograph of histomorphologic evaluation of lymphoid organs”. Toxicology Pathology 34 (2006): 409-496.
  17. Zhang R., et al. “Systemic immune system alterations in early stages of Alzheimer’s disease”. Journal of Neuroimmunology 256 (2013): 38-42.
  18. Hovden H., et al. “Immune system alterations in amyotrophic lateral sclerosis”. Acta Neurologica Scandinavica 128 (2013): 287-296.
  19. NTP (National Toxicology Program). Toxicology and carcinogenesis studies of toluene (CAS No. 108-88-3) in F344/N rats and B5C3F1 mice (inhalation studies). Public Health Service, U.S. Department of Health and Human Services; NTP TR 371 (1990).
  20. Huerta-Rivas A., et al. “Toluene impairs learning and memory, has antinociceptive effects, and modifies histone acetylation in the dentate gyrus of adolescent and adult rats”. Pharmacology Biochemistry and Behavior 102 (2012): 48-57.
  21. Huff J. “Absence of carcinogenic activity in Fischer rats and B6C3F1 mice following 103-week inhalation exposures to toluene”. International Journal of Occupational and Environmental Health 9 (2003): 138-146.
  22. Win-Shwe T-T., et al. “Developmental stage-specific changes in immunological biomarkers in male C3H/HeN mice after early life toluene exposure”. Toxicology Letters 208 (2012): 133-141.
  23. Fujimaki H., et al. “Dysregulation of immune responses in an allergic mouse model following low-level toluene exposure”. Toxicology 286 (2011): 28-35.
  24. Yamamoto S., et al. “Suppression of Th1- and Th2-type immune responses in infant mouse spleen after prenatal and postnatal exposure to low-level toluene and peptidoglycan”. Inhalation Toxicology 9 (2009): 793-802.
  25. Spoor MS., et al. “Characterization of age- and gender-related changes in the spleen and thymus from control cynomolgus macaques used in toxicity studies”. Toxicologic Pathology 36 (2008): 695-704.
  26. Frouin H., et al. “Toxic effects of various pollutants in 11B7501 lymphoma B cell line from harbour seal (Phocavitulina)”. Toxicology 2-3 (2010): 66-76.
  27. Bencherifa M., et al. “Alpha7 nicotinic cholinergic neuromodulation may reconcile multiple neurotransmitter hypotheses of schizophrenia”. Medical Hypotheses 78 (2012): 594-600.
  28. Filley CM. “Toluene abuse and white matter: a model of toxic leukoencephalopathy”. Psychiatric Clinics of North America 36 (2013): 293-302.
  29. Bouronikou E., et al. “Metabolism-related cytokine and hormone levels in the serum of patients with myelodysplastic syndromes”. Acta Haematologica 1 (2013): 27-33.
  30. Miyake S. “Mind over cytokines: Crosstalk and regulation between the neuroendocrine and immune systems”. Clinical and Experimental Neuroimmunology 3 (2012): 1-15.
  31. Taub DD., et al. “Rejuvenation of the aging thymus: growth hormone-mediated and ghrelin-mediated signaling pathways”. Current Opinion in Pharmacology 10 (2010): 408-424.
  32. Jara LJ., et al. “Prolactin and autoimmunity”. Clinical Reviews in Allergy and Immunology 40 (2011): 50-59.
  33. Pállinger É., et al. “Changes in the hormone (ACTH, insulin, epinephrine) content of immune cells in children having acute lymphocytic leukemia (All)”. Acta Microbiologica et Immunologica Hungarica 60 (2013): 423-431.
Citation: Vladimir Nikolaevich Voloshin., et al.“ Spleen Organometry of Old Male Albino Rats After Whole-Body Exposure to Toluene”. EC Clinical and Experimental Anatomy 3.2 (2020): 01-08.

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