Research Article
Volume 3 Issue 2 - 2020
"Spleen Organometry of Old Male Albino Rats After Whole-Body Exposure to Toluene"
Vladimir Nikolaevich Voloshin1*, Irina Sergeevna Voloshina2, Dmitry Alekseevich Lugovskov3, Alexander Nikolaevich Nesterenko3, Ida Yakovlevna Kozhemyaka1 and Maxim Gennadievich Grischuk4
1Department of Human Anatomy, St. Luke Lugansk State Medical University, Ukraine
2Department of Radiology, St. Luke Lugansk State Medical University, Ukraine
3Department of Stomatology, St. Luke Lugansk State Medical University, Ukraine
4Department of Pathological Physiology, St. Luke Lugansk State Medical University, Ukraine
*Corresponding Author:Vladimir Nikolaevich Voloshin, Department of Human Anatomy, St. Luke Lugansk State Medical University, Ukraine.
Received: November 20, 2019; Published: January 07, 2020




Abstract

Spleen is a secondary lymphoid organ that is highly sensitive to different chemicals. Toluene is an aromatic hydrocarbon commonly used as an industrial solvent and can be considered as a potential immunotoxin. We aimed to investigate the organometry of the spleen from rats exposed to toluene. The toxicity of toluene was evaluated in male albino rats via whole-body exposure. The animals were exposed to target concentrations of 0 (air control) and 133 ppm of toluene in air for 5 hours/day, 5 days/week, for 2 month. The animals were weighted and decapitated at different time points (one, seven, sixteen, thirty one and sixty one day) post-exposure. The weight, length, width and thickness of the spleen were measured. It was studied absolute and relative weight of the spleen.

Rats that were withdrawn from the experiment 30 days after the cessation of toluene had an organ weight of 1134.67 mg. This indicator was not statistically significantly higher than the data of the respective control group by 5.75% (p = 0.109). The weight of the organ of rats of group 5 of series T was at the level of 1217.33 mg, which was 103.11% (p = 0.222) to the control. Rats that were withdrawn from the experiment 7 and 15 an organ length of 41.64 mm and 43.04 mm, respectively. In the 4 and 5 groups of rats, the average length of the organ was 104.71% (p = 0.076) and 97.82% (p = 0.282) to the data of the corresponding groups of the control series. Rats withdrawn from the experiment 15 and 30 days after toluene cessation had an organ thickness of 4.91 mm and 4.97 mm, which was 100.40% (p = 0.729) and 103.11% (p = 0.927) to the control indicators. The results demonstrate that inhaled toluene does not significantly affect the weight and size of the spleen of old rats.

Keywords: Toluene; Toxicity; Rat; Spleen

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Citation: Vladimir Nikolaevich Voloshin., et al.“ Spleen Organometry of Old Male Albino Rats After Whole-Body Exposure to Toluene”. EC Clinical and Experimental Anatomy 3.2 (2020): 01-08.

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