Research Article
Volume 3 Issue 12 - 2020
Histo-Immune Expression of Stem Cell Populations in Mouse Thyroid Gland Against Age Changes and the Possible Protective Effect of Selenium
Laila M Aboul- Mahasen1,4*, Mai M Ashgan2 and Saleh A AlKarim2,3
1Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia
2Biology Departments, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
3Embryonic and cancer stem cells research group, king Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia
4Anatomy Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt
*Corresponding Author: Laila M Aboul- Mahasen, Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
Received: November 17, 2020; Published: November 30, 2020




Abstract

This study evaluated the histological structures of mouse thyroid gland and the immunohistochemical expression of thyroid differentiation marker (Tg) and stem cell marker (P63) with special reference to the site and degree of expression pattern of stem cells in the mouse thyroid gland in related to age and the possible prophylactic effect of selenium from development of aging-associated stem cell defects.

Material and Methods: Sixty laboratory mice were divided into six groups at ages of (1 day, 15day, 30 day, 2 - 3 months, 12 - 25 months, 12 - 24 months old and 12 - 24 months treated with 5 mcg selenium for 20 successive days. After finishing the experiment, blood samples were collected from all groups for assessment of thyroid function tests (T3 and T4). Further, data collected from the experiment were analyzed statistically.

Results: A gradual increase of T3 and T4 with age and a significant decrease of T3 and T4 at the old age was evaluated. At selenium treated group, T3 and T4 levels were significantly increased again.

Histological development of thyroid glands till old age were studied. The active follicles contained various amounts of colloid and were arranged with one layer of cuboidal or simple columnar cells. The production of thyroglobulin by the active follicles was detected by the strong Tg expression. In the old age, most of thyroid follicles were inactive, appeared empty or contained accumulated colloid and were arranged with a single layer or multiple layers of simple cuboidal or squamous cells. Areas of hyperplastic changes which might be follicular or C- cell hyperplasia were detected. In the present study, stem cells were detected as two populations of cells called solid cell nests (SCNs) and side populations (SP) or as solitary scattered cells. There were also mixed solid SCNs (cystic SCNs). The SP cells and the SCNs gave negative Tg expression, while the nuclei of the SP and nuclei of the main cells of SCNs gave strong P63 expression indicating the stem cells like – characters. The C-Cells gave negative Tg and P63 expression.

Conclusion: From the present study, it could be concluded that the stem cells were P63 expressed in all groups even at the old age but in the old age, P63 was expressed in few populations of cells and few solitary scattered cells indicating the presence of stem cells at old age but with less amount.The stem cells of selenium treated aged group showed a better pattern of P63 expression as that of the adult age indicating the activation of the silent aged stem cells and restoration of their activity.

Recommendations: Selenium daily supplementation in old age is recommended for protection from physiological senile hypothyroidism and improvement of the thyroid function.

Keywords: Histo-Immune Expression; Cell Populations

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Citation: Laila M Aboul- Mahasen., et al. “Histo-Immune Expression of Stem Cell Populations in Mouse Thyroid Gland Against Age Changes and the Possible Protective Effect of Selenium”. EC Clinical and Experimental Anatomy 3.12 (2020): 37-51.

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