Research Article
Volume 3 Issue 12 - 2020
Histo-Immune Expression of Stem Cell Populations in Mouse Thyroid Gland Against Age Changes and the Possible Protective Effect of Selenium
Laila M Aboul- Mahasen1,4*, Mai M Ashgan2 and Saleh A AlKarim2,3
1Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia
2Biology Departments, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
3Embryonic and cancer stem cells research group, king Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia
4Anatomy Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt
*Corresponding Author: Laila M Aboul- Mahasen, Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
Received: November 17, 2020; Published: November 30, 2020


This study evaluated the histological structures of mouse thyroid gland and the immunohistochemical expression of thyroid differentiation marker (Tg) and stem cell marker (P63) with special reference to the site and degree of expression pattern of stem cells in the mouse thyroid gland in related to age and the possible prophylactic effect of selenium from development of aging-associated stem cell defects.

Material and Methods: Sixty laboratory mice were divided into six groups at ages of (1 day, 15day, 30 day, 2 - 3 months, 12 - 25 months, 12 - 24 months old and 12 - 24 months treated with 5 mcg selenium for 20 successive days. After finishing the experiment, blood samples were collected from all groups for assessment of thyroid function tests (T3 and T4). Further, data collected from the experiment were analyzed statistically.

Results: A gradual increase of T3 and T4 with age and a significant decrease of T3 and T4 at the old age was evaluated. At selenium treated group, T3 and T4 levels were significantly increased again.

Histological development of thyroid glands till old age were studied. The active follicles contained various amounts of colloid and were arranged with one layer of cuboidal or simple columnar cells. The production of thyroglobulin by the active follicles was detected by the strong Tg expression. In the old age, most of thyroid follicles were inactive, appeared empty or contained accumulated colloid and were arranged with a single layer or multiple layers of simple cuboidal or squamous cells. Areas of hyperplastic changes which might be follicular or C- cell hyperplasia were detected. In the present study, stem cells were detected as two populations of cells called solid cell nests (SCNs) and side populations (SP) or as solitary scattered cells. There were also mixed solid SCNs (cystic SCNs). The SP cells and the SCNs gave negative Tg expression, while the nuclei of the SP and nuclei of the main cells of SCNs gave strong P63 expression indicating the stem cells like – characters. The C-Cells gave negative Tg and P63 expression.

Conclusion: From the present study, it could be concluded that the stem cells were P63 expressed in all groups even at the old age but in the old age, P63 was expressed in few populations of cells and few solitary scattered cells indicating the presence of stem cells at old age but with less amount.The stem cells of selenium treated aged group showed a better pattern of P63 expression as that of the adult age indicating the activation of the silent aged stem cells and restoration of their activity.

Recommendations: Selenium daily supplementation in old age is recommended for protection from physiological senile hypothyroidism and improvement of the thyroid function.

Keywords: Histo-Immune Expression; Cell Populations


    1. Di Lauro R and De Felice M. “Thyroid gland: anatomy and development”. In: De Groot, L.; Jameson, J., editors. Endocrinology. Philadelphia: Saunders (2001): 1268-1278.
    2. Dumont JE., et al. “Physiological and pathological regulation of thyroid cell proliferation and differentiation by thyrotropin and other factors”. Physiological Reviews 72 (1992): 667-697.
    3. Cameselle -Teijeiro J., et al. “Solid cell nests of the thyroid: light microscopyand immunohistochemical”. Human Pathology 7 (1994): 684-693.
    4. Harach HR. “Solid cell nests of the thyroid”. The Journal of Pathology 155 (1988): 191-200.
    5. Davies TF., et al. “The emerging cell biology of thyroid stem cells”. The Journal of Clinical Endocrinology and Metabolism 96 (2011): 2692-2702.
    6. Moreno MJR., et al. “Immunohistochemical profile of solid cell nest of thyroid gland”. Endocrine Pathology 22 (2011): 35-39.
    7. Thomas T., et al. “Expression of Endoderm Stem Cell Markers: Evidence for the Presence of Adult Stem Cells in Human Thyroid Glands”. Thyroid 6 (2006): 537-544.
    8. Hoshi N., et al. “Side population cells in the mouse thyroid exhibit stem/progenitor cell-like characteristics”. Endocrinology 9 (2007): 4251-4258.
    9. Juhyun OH., et al. “Stem cell aging: mechanisms, regulators and therapeutic opportunities”. Nature Medicine 8 (2014): 870-880.
    10. Ahmed ASI., et al. “Effect of aging on stem cells”. World Journal of Experimental Medicine 1 (2017): 1-10.
    11. Kohrle JR and Gartner R. “Selenium and thyroid”. Best Practice and Research Clinical Endocrinology and Metabolism 23 (2009): 815-827.
    12. Schomburg L. “Selenium, selenoproteins and the thyroid gland”. Nature Reviews Endocrinology 3 (2011): 160-171.
    13. Drury RA and Wallington EA. “Carleton’s Histological Technique”. edition.7, Oxford University Press, New York, Toronto (1980): 137-145.
    14. Rayman MP. “The argument for increasing selenium intake”. Proceedings of the Nutrition Society 2 (2002): 203-215.
    15. Nair AB and Jacob S. “A simple practice guide for dose conversion between animals and human”. Journal of Basic and Clinical Pharmacy 227 (2016): 31.
    16. Leung A. “Thyroid Emergencies”. Journal of Infusion Nursing5 (2016): 281-286.
    17. Yue L., et al. “Change of peripheral tissue thyroid hormone metabolism in rats fed with selenium and vitamin E deficient artificial semisynthetic diet”. Chinese Medicine 9 (1998): 854-857.
    18. Pekar JJ., et al. “Effect of selenium supplementation in thyroid gland diseases”. Journal of Elementology 1 (2015): 91-103.
    19. Ohida S. “Histological studies of thyroid gland of the mouse after castration Okajimas”. Folia Anat J pn.5-6 (1954): 347-370.
    20. Lee J., et al. “Morphological and Functional Changes in the Thyroid Follicles of the Aged Murine and Humans”. Journal of Pathology and Translational Medicine 50 (2016): 426-435.
    21. Rao- Rupanagdi S., et al. “Age-related Changes in Thyroid Structure and Function in Sprague-Dawley Rats”. Veterinary Pathology 29 (1992): 278-287.
    22. Igbokwe CO and Ezeasor DN. “Histological and immunohistochemical changes of the thyroid gland during the foetal and postnatal period of development in indigenous large white crossbred pigs”. Bulgarian Journal of Veterinary Medicine4 (2015): 313-324.
    23. Gartner LP and Hiatt J. “Color Atlas and Textbook of Histology”. edition 6. Wolters Kluwer , Lippincott. Williams and Wilkins (2014): 232.
    24. Scognamiglio T. “C Cell and Follicular Epithelial Cell Precursor Lesions of the Thyroid”. Archives of Pathology and Laboratory Medicine12 (2017): 1646-1652.
    25. Ma R., et al. “Thyroid Follicle Formation and Thyroglobulin Expression in Multipotent Endodermal Stem Cells”. Thyroid 4 (2013).
    26. Reis-Filho JS., et al. “P63 expression in solid cell nests of the thyroid :further evidence for a stem cell origin”. Modern Pathology 1 (2003): 43-48.
    27. Burstein DE., et al. “Immunohistochemical detection of p53 homolog p63 in solid cell nests, papillary thyroid carcinoma, and Hashimoto’s Thyroiditis: a stem cell hypothesis of papillary carcinoma oncogenesis”. Human Pathology 4 (2004): 465-473.
    28. Kimura S. “Thyroid regeneration: how stem cells play a role?” Endocrinology55 (2014): 1-9.
    29. Mitsutake N., et al. “Characterization of Side Population in Thyroid Cancer Cell Lines: Cancer Stem-Like Cells Are Enriched Partly But Not Exclusively”. Endocrinology4 (2007): 1797-1803.
    30. Luca AH. “Squamous cell differentiation in metastatic papillary thyroid carcinoma: metaplastic reversion or progression”. Case Report Iranian Journal of Pathology 2 (2018): 276-280.
    31. Fukumoto J., et al. “The expansion of p63- positive CCSP-positive epithelial cells and altered expressions of p63 isoforms in the lung as novel features of aging”. American Journal of Physiology - Cell Physiology (2019): 492-508.
    32. Zeng W., et al. “Antioxidant treatment enhances human mesenchymal stem cell anti-stress ability and therapeutic efficacy in an acute liver failure model”. Scientific Reports 5 (2015): 11100.
    33. Brezinska S. “Fever induced oxidative stress: the effect on thyroid status and the 5- monodeiodinase activity, productive role of selenium and vitamins”. The European Journal of Pharmacology 2 (2001): 275-284.
    34. Ventura M., et al. “Selenium and Thyroid Disease: From Pathophysiology to Treatment”. International Journal of Endocrinology (2017).
    35. Han SA., et al. “Selenium increases proliferation, migration and stemness genes expression in human synovium derived stromal cells. Poster No. 980 • 55th Annual Meeting of the Orthopaedic Research Society) (2009).
    36. Park SH., et al. “Selenium improves stem cell potency by stimulating the proliferation and active migration of 3T3-L1 preadipocytes”. International Journal of Oncology 44 (2014): 336-342.
    37. Jobeili L., et al. “Selenium preserves keratinocyte stemness and delays senescence by maintaining epidermal adhesion”. Aging11 (2017): 2302-2315.
Citation: Laila M Aboul- Mahasen., et al. “Histo-Immune Expression of Stem Cell Populations in Mouse Thyroid Gland Against Age Changes and the Possible Protective Effect of Selenium”. EC Clinical and Experimental Anatomy 3.12 (2020): 37-51.

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