Research Article
Volume 4 Issue 1 - 2021
Comparative Study: Topical Application of Zinc Oxide and Low-Level Laser Therapy in Subcutaneous Wound Repair of Rats
Fahime Oukati Sadegh1, Muhammad Asif2, Muhammad Riaz Baig Chughtai3, Hina Khan4*, Vinod Kumar5 and Nouman Issani5
1Ofogh Physiotherapy Clinical, Mashhad, Iran
2Professor, Isra Institute of Rehabilitation Sciences, Karachi, Pakistan
3Principal, College of Physical therapy, JPMC, Karachi, Pakistan
4Associate Professor of Anatomy, Deputy Director Research and PG Affairs, Al-Tibri Medical College and Hospital, Karachi, Pakistan
5Assistant Professor, Isra Institute of Rehabilitation Sciences, Karachi, Pakistan
*Corresponding Author: Hina Khan, Associate Professor of Anatomy, Deputy Director Research and PG Affairs, Al-Tibri Medical College and Hospital, Karachi, Pakistan.
Received: October 27, 2020; Published: November 29, 2020


Objective of the Study: To evaluate the Healing effects with topical application of zinc oxide and Low laser therapy in subcutaneous wound of rats.

Study Design: Experimental study.

Place and Duration of the Study: The study was done from March 2018 to November 2018 at Isra Institute of rehabilitation Sciences, Isra University Karachi Campus.

Materials and Methods: After taken an ethical consideration of concerned institute, total 27 numbers of male rats were selected on the basis of randomized sampling. Total numbers of male albino rats with a weight between 150 to 250 gms were equally divided into three groups based on the topical application of therapeutic agents. Group A (Control Group) received normal saline topically once daily. Group B was given a topical application of 20% Zinc oxide once daily for 14 days. Group C was subjected to Low-level laser therapy 4 j/cm2 once daily for 30 seconds. The wound was created on the dorsal surface of rats 2 x 2 cm2 after giving anesthesia with ethanol, and the size of the wound measured with the help of normal scale. For assessment, the photograph of the wound was taken by a DSLR camera. Data were analyzed by applying One-way ANOVA to evaluate the differences between the Mean of wound size among the different groups. P =0.05 took the level of significance.

Results: Low-level Laser Therapy showed rapid healing effects as compared to zinc oxide with significant anti-inflammatory response.

Conclusion: Low-level Laser Therapy evidenced the rapid healing effects on different days of sampling as compared to other therapeutic groups.

Keywords: Wound; Zinc Oxide; Low-Level-Laser


    1. Martin P. “Wound healing--aiming for perfect skin regeneration”. Science5309 (1997): 75-81.
    2. Hu L., et al. “Herbal medicines that benefit epidermal permeability barrier function”. Dermatologica Sinica2 (2015): 90-95.
    3. Baker OJ. “Current trends in salivary gland tight junctions”. Tissue Barriers3 (2016): e1162348.
    4. Bin Mh Busra MF., et al. “Tissue-engineered skin substitute enhances wound healing after radiation therapy”. Advances in Skin and Wound Care 3 (2016): 120-129.
    5. Wan W., et al. “A skin-inspired 3D bilayer scaffold enhances granulation tissue formation and anti-infection for diabetic wound healing”. Journal of Materials Chemistry B18 (2019): 2954-2961.
    6. Olingy CE., et al. “Non-classical monocytes are biased progenitors of wound healing macrophages during soft tissue injury”. Scientific Reports1 (2017): 447.
    7. Demir H., et al. “Comparison of the effects of laser, ultrasound, and combined laser+ ultrasound treatments in experimental tendon healing”. Lasers in Surgery and Medicine: The Official Journal of the American Society for Laser Medicine and Surgery1 (2004): 84-89.
    8. Wardlaw JL., et al. “Laser therapy for incision healing in 9 dogs”. Frontiers in Veterinary Science 5 (2019): 349.
    9. Livingstone C. “Zinc: physiology, deficiency, and parenteral nutrition”. Nutrition in Clinical Practice3 (2015): 371-382.
    10. Mohammed J., et al. “Severe infant rash resistant to therapy due to zinc deficiency”. Pediatric Emergency Care8 (2017): 582-584.
    11. De Medeiros ML., et al. “Effect of low-level laser therapy on angiogenesis and matrix metalloproteinase-2 immunoexpression in wound repair”. Lasers in Medical Science1 (2017): 35-43.
    12. Ahmed OM., et al. “Quercetin and low level laser therapy promote wound healing process in diabetic rats via structural reorganization and modulatory effects on inflammation and oxidative stress”. Biomedicine and Pharmacotherapy 101 (2018): 58-73.
    13. Lacjaková K., et al. “Effects of equal daily doses delivered by different power densities of low-level laser therapy at 670 nm on open skin wound healing in normal and corticosteroid-treated rats: a brief report”. Lasers in Medical Science5 (2010): 761-766.
    14. Reis SR., et al. “Effect of 670-nm laser therapy and dexamethasone on tissue repair: a histological and ultrastructural study”. Photomedicine and Laser Surgery4 (2008): 307-313.
    15. Abdullah BJ., et al. “Evaluate the effects of platelet rich plasma (PRP) and zinc oxide ointment on skin wound healing”. Annals of Medicine and Surgery 37 (2019): 30-37.
    16. Lin PH., et al. “Zinc in wound healing modulation”. Nutrients1 (2018): 16.
    17. Chhabra H., et al. “A nano zinc oxide doped electrospun scaffold improves wound healing in a rodent model”. RSC Advances2 (2016): 1428-1439.
    18. Xu Q., et al. “Zinc ion coordinated poly (ionic liquid) antimicrobial membranes for wound healing”. ACS Applied Materials and Interfaces17 (2017): 14656-1464.
Citation: Hina Khan., et al. “Comparative Study: Topical Application of Zinc Oxide and Low-Level Laser Therapy in Subcutaneous Wound Repair of Rats”. EC Clinical and Experimental Anatomy 4.1 (2020): 17-21.

PubMed Indexed Article

EC Pharmacology and Toxicology
LC-UV-MS and MS/MS Characterize Glutathione Reactivity with Different Isomers (2,2' and 2,4' vs. 4,4') of Methylene Diphenyl-Diisocyanate.

PMID: 31143884 [PubMed]

PMCID: PMC6536005

EC Pharmacology and Toxicology
Alzheimer's Pathogenesis, Metal-Mediated Redox Stress, and Potential Nanotheranostics.

PMID: 31565701 [PubMed]

PMCID: PMC6764777

EC Neurology
Differences in Rate of Cognitive Decline and Caregiver Burden between Alzheimer's Disease and Vascular Dementia: a Retrospective Study.

PMID: 27747317 [PubMed]

PMCID: PMC5065347

EC Pharmacology and Toxicology
Will Blockchain Technology Transform Healthcare and Biomedical Sciences?

PMID: 31460519 [PubMed]

PMCID: PMC6711478

EC Pharmacology and Toxicology
Is it a Prime Time for AI-powered Virtual Drug Screening?

PMID: 30215059 [PubMed]

PMCID: PMC6133253

EC Psychology and Psychiatry
Analysis of Evidence for the Combination of Pro-dopamine Regulator (KB220PAM) and Naltrexone to Prevent Opioid Use Disorder Relapse.

PMID: 30417173 [PubMed]

PMCID: PMC6226033

EC Anaesthesia
Arrest Under Anesthesia - What was the Culprit? A Case Report.

PMID: 30264037 [PubMed]

PMCID: PMC6155992

EC Orthopaedics
Distraction Implantation. A New Technique in Total Joint Arthroplasty and Direct Skeletal Attachment.

PMID: 30198026 [PubMed]

PMCID: PMC6124505

EC Pulmonology and Respiratory Medicine
Prevalence and factors associated with self-reported chronic obstructive pulmonary disease among adults aged 40-79: the National Health and Nutrition Examination Survey (NHANES) 2007-2012.

PMID: 30294723 [PubMed]

PMCID: PMC6169793

EC Dental Science
Important Dental Fiber-Reinforced Composite Molding Compound Breakthroughs

PMID: 29285526 [PubMed]

PMCID: PMC5743211

EC Microbiology
Prevalence of Intestinal Parasites Among HIV Infected and HIV Uninfected Patients Treated at the 1o De Maio Health Centre in Maputo, Mozambique

PMID: 29911204 [PubMed]

PMCID: PMC5999047

EC Microbiology
Macrophages and the Viral Dissemination Super Highway

PMID: 26949751 [PubMed]

PMCID: PMC4774560

EC Microbiology
The Microbiome, Antibiotics, and Health of the Pediatric Population.

PMID: 27390782 [PubMed]

PMCID: PMC4933318

EC Microbiology
Reactive Oxygen Species in HIV Infection

PMID: 28580453 [PubMed]

PMCID: PMC5450819

EC Microbiology
A Review of the CD4 T Cell Contribution to Lung Infection, Inflammation and Repair with a Focus on Wheeze and Asthma in the Pediatric Population

PMID: 26280024 [PubMed]

PMCID: PMC4533840

EC Neurology
Identifying Key Symptoms Differentiating Myalgic Encephalomyelitis and Chronic Fatigue Syndrome from Multiple Sclerosis

PMID: 28066845 [PubMed]

PMCID: PMC5214344

EC Pharmacology and Toxicology
Paradigm Shift is the Normal State of Pharmacology

PMID: 28936490 [PubMed]

PMCID: PMC5604476

EC Neurology
Examining those Meeting IOM Criteria Versus IOM Plus Fibromyalgia

PMID: 28713879 [PubMed]

PMCID: PMC5510658

EC Neurology
Unilateral Frontosphenoid Craniosynostosis: Case Report and a Review of the Literature

PMID: 28133641 [PubMed]

PMCID: PMC5267489

EC Ophthalmology
OCT-Angiography for Non-Invasive Monitoring of Neuronal and Vascular Structure in Mouse Retina: Implication for Characterization of Retinal Neurovascular Coupling

PMID: 29333536 [PubMed]

PMCID: PMC5766278

EC Neurology
Longer Duration of Downslope Treadmill Walking Induces Depression of H-Reflexes Measured during Standing and Walking.

PMID: 31032493 [PubMed]

PMCID: PMC6483108

EC Microbiology
Onchocerciasis in Mozambique: An Unknown Condition for Health Professionals.

PMID: 30957099 [PubMed]

PMCID: PMC6448571

EC Nutrition
Food Insecurity among Households with and without Podoconiosis in East and West Gojjam, Ethiopia.

PMID: 30101228 [PubMed]

PMCID: PMC6086333

EC Ophthalmology
REVIEW. +2 to +3 D. Reading Glasses to Prevent Myopia.

PMID: 31080964 [PubMed]

PMCID: PMC6508883

EC Gynaecology
Biomechanical Mapping of the Female Pelvic Floor: Uterine Prolapse Versus Normal Conditions.

PMID: 31093608 [PubMed]

PMCID: PMC6513001

EC Dental Science
Fiber-Reinforced Composites: A Breakthrough in Practical Clinical Applications with Advanced Wear Resistance for Dental Materials.

PMID: 31552397 [PubMed]

PMCID: PMC6758937

EC Microbiology
Neurocysticercosis in Child Bearing Women: An Overlooked Condition in Mozambique and a Potentially Missed Diagnosis in Women Presenting with Eclampsia.

PMID: 31681909 [PubMed]

PMCID: PMC6824723

EC Microbiology
Molecular Detection of Leptospira spp. in Rodents Trapped in the Mozambique Island City, Nampula Province, Mozambique.

PMID: 31681910 [PubMed]

PMCID: PMC6824726

EC Neurology
Endoplasmic Reticulum-Mitochondrial Cross-Talk in Neurodegenerative and Eye Diseases.

PMID: 31528859 [PubMed]

PMCID: PMC6746603

EC Psychology and Psychiatry
Can Chronic Consumption of Caffeine by Increasing D2/D3 Receptors Offer Benefit to Carriers of the DRD2 A1 Allele in Cocaine Abuse?

PMID: 31276119 [PubMed]

PMCID: PMC6604646

EC Anaesthesia
Real Time Locating Systems and sustainability of Perioperative Efficiency of Anesthesiologists.

PMID: 31406965 [PubMed]

PMCID: PMC6690616

EC Pharmacology and Toxicology
A Pilot STEM Curriculum Designed to Teach High School Students Concepts in Biochemical Engineering and Pharmacology.

PMID: 31517314 [PubMed]

PMCID: PMC6741290

EC Pharmacology and Toxicology
Toxic Mechanisms Underlying Motor Activity Changes Induced by a Mixture of Lead, Arsenic and Manganese.

PMID: 31633124 [PubMed]

PMCID: PMC6800226

EC Neurology
Research Volunteers' Attitudes Toward Chronic Fatigue Syndrome and Myalgic Encephalomyelitis.

PMID: 29662969 [PubMed]

PMCID: PMC5898812

EC Pharmacology and Toxicology
Hyperbaric Oxygen Therapy for Alzheimer's Disease.

PMID: 30215058 [PubMed]

PMCID: PMC6133268

News and Events

December Issue Release

We always feel pleasure to share our updates with you all. Here, notifying you that we have successfully released the November issue of respective journals and the latest articles can be viewed on the current issue pages.

Submission Deadline for Upcoming Issue

ECronicon delightfully welcomes all the authors around the globe for effective collaboration with an article submission for the upcoming issue of respective journals. Submissions are accepted on/before December 20, 2022.

Certificate of Publication

ECronicon honors with a "Publication Certificate" to the corresponding author by including the names of co-authors as a token of appreciation for publishing the work with our respective journals.

Best Article of the Issue

Editors of respective journals will always be very much interested in electing one Best Article after each issue release. The authors of the selected article will be honored with a "Best Article of the Issue" certificate.

Certifying for Review

ECronicon certifies the Editors for their first review done towards the assigned article of the respective journals.

Latest Articles

The latest articles will be updated immediately on the articles in press page of the respective journals.