Research Article
Volume 3 Issue 3 - 2020
Gene and Marker Stem Cells Expression in Mouse Thyroid Gland against Age Changes and the Prophylactic Role of Selenium
Laila M Aboul-Mahasen Morsy1,4*, Mai M Ashgan2 and Saleh A AlKarim2,3
11Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia
22Biology Departments, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
33Embryonic and Cancer Stem Cells Research Group, king Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia
44Anatomy Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt
*Corresponding Author: Laila M Aboul-Mahasen Morsy, Professor, Anatomy Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia and Anatomy Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.
Received: March 12, 2020; Published: April 10, 2020




Abstract

Background/Aim: The expression of stem cells in developing and aged thyroid tissue is still not clearly detected. This study is aimed to investigate the stem cell expression in mouse thyroid gland at different ages from newborn to old age and the possible prophylactic effect of selenium from development of aging-associated stem cell defects.

Materials and Methods: 60 laboratory mice were used and divided into six groups at ages of (one day, 15 days, 30 days, 2 - 3 months, 12 - 24 months and selenium treated 12 - 24 months). The treated group were given selenium (5 mcg) orally for 20 successive days. Blood were harvested from all groups for assessment of thyroid function tests (TSH, T3 and T4). Slices of thyroid glands were prepared for P63 immunohistochemistry and for qRT-PCR gene expression pattern for genes ABCG2, GNL3, OCT4 and paired box PAX8 genes against reference actin beta (ACTB) in mice. Real time quantitative PCR data were analyzed by Livak method.

Results: The immunohistochemical and RT-qPCR results revealed gene expression of stem cells in all ages but with lower expression in aged group than those in adult group. In the aged group treated with selenium, the stem cells expression is improved. Relative quantification for OCT4 and the fold changes in comparative differential expression revealed a significant upregulation only in the aged group. However, gene expression in the selenium treated aged group gave nearly the same expression as those of the adult and 30 days old groups.

Conclusion: As the biochemical results in selenium treated aged group confirmed the immunohistochemical and RT-qPCR gene expression results, So, it could be concluded that the stem cells have been expressed in the mouse thyroid tissues at all ages and the possible prophylactic effect of selenium on the aged stem cells had been detected.

Keywords: Stem Cells; Postnatal Developing Thyroid Tissue; Aging Process; Selenium; Reference Gene (ACTB); Stem Cell Marker P63; ABCG2; GNL3; OCT4; PAX8 Genes

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Citation: Laila M Aboul-Mahasen Morsy., et al. “Gene and Marker Stem Cells Expression in Mouse Thyroid Gland against Age Changes ctic Role of Selenium". EC Clinical and Experimental Anatomy 3.5 (2020): 05-21.

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