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Research Article
Volume 1 Issue 1 - 2015
Helicobacter Pylori Infection and Its Potential Role in Childhood Eczema
Abdelrazak ali1,2*, Ayman MN3 and Mahmoud MA4
1Department of Pediatrics, Al-Azhar University, Cairo, Egypt
2Last place of work, Saudi Arabia, Dr. Noor Mohammad Khan General Hospital
3Department of Pharmacy Practice, Chapman University, Jeronimo RdSte, Irvine, CA
4Consultant of Hospital management and Medical Quality Control, Egypt
*Corresponding Author: Abdelrazak ali, Department of Pediatrics, Al-Azhar University, Cairo, Egypt.
Received: November 01, 2015; Published: November 13, 2015
Citation: Abdelrazak ali., et al. “Helicobacter Pylori Infection and Its Potential Role in Childhood Eczema”. EC Bacteriology and Virology Research 1.1 (2015): 5-12.
Objective: To determine whether Helicobacter pylori is associated with childhood eczema.
Design: Case-control study.
Settings: Al Azhar University Hospitals in Cairo, Egypt. Local hospital in Hafer Al Batin, Saudi Arabia.
Participants: A total of 170 patients with Atopic dermatitis (Eczema) who were 2 months to 7 years old and had fulfilled the American Academy of Dermatology Criteria for Atopic dermatitis (AD), and a total of 80 healthy controls with no history of Atopy were matched by country of origin, age, sex, family size, socio- demographic variables and ethnicity to the 170 atopic cases.
Main Outcome Measure: Helicobacter pylori infection determined by H pylori stool antigen testing and serologicanti-H. pylori IgG antibodies.
Results: Of the 170 patients presenting with atopic dermatitis, 6 cases (3.5%) tested positive serologicanti-H. pylori IgG antibodies and 12 cases (7.0%) tested positive H pylori Stool antigen; Of the 80 healthy controls, 12 cases (15.0%), 6cases (7.5.%) and 2case (2.5%) were tested positive by serology, stool antigen and both Serology/Stool antigen tests respectively. (For serology testing, Odds Ratio = 0.2073, 95% CI = 0.0748 - 0.5749, Z statistics = 3.024, P value = 0.0025).
Conclusion: H pylori infection is associated with childhood eczema in genetically predisposed atopic children. Significant inverse correlation between atopic dermatitis and positive H pylori serologic testing was reported.
Keywords: Helicobacter pylori; Atopic eczema; Atopic dermatitis; Helminthes
Atopic dermatitis (AD) also known as atopic eczema or eczema [1] is a type of dermatitis, an inflammatory, relapsing, non-contagious and itchy skin disorder [2]. It is often chronic in nature. In children under one year of age much of the body may be affected. As they get older the back of the knees and front of the elbows are the most common area for the rash. In adults the hands and feet are the most affected [3]. The cause is believed to involve a number of factors including; genetics, environmental exposures, and difficulties in permeability of the skin. The diagnosis is based on the signs and symptoms. Other diseases that need to be excluded are contact dermatitis, psoriasis and seborrheic dermatitis [3]. The ISAAC (International Study of Asthma and Allergies in Childhood) revealed that AD affects children all over the world, although the disease prevalence varies substantially among countries [4]. The prevalence of AD is also increasing, especially in developing countries [5]. It affects more than 10% of children in the United States and is more common in younger children. Most people outgrow it [3]. It has been given names like “prurigoBesnier,” “neurodermatitis,” “endogenous eczema,” “flexural eczema,” “infantile eczema,” and “prurigodiathesique”[6]. Since the beginning of the twentieth century, many mucosal inflammatory disorders have become more common; atopic eczema (AE) is a classic example of such disease. It now affects 15–30% of children and 2–10% of adults in developed countries. In the United States it has nearly tripled in the past thirty to forty years [7]. Prevalence continues to vary and has changed in different regions of the world. Nigeria, the United Kingdom and New Zealand had been areas of the highest prevalence. The prevalence of AD seems to have reached a plateau around 20% in countries with the highest prevalence, suggesting that AD may not be on a continued rise but that a finite number of individuals may be susceptible to the condition. Risk factors associated with increased prevalence include higher socioeconomic status, higher level of family education, smaller family size and urban environment [8]. Research indicates that food allergy and atopic sensitization to environmental allergens may not be directly causal of the condition and that a non-atopic form of the condition exists. About 60% of patients will experience remission. The number of patients who will progress through the atopic march to develop asthma and allergic rhinitis depends on the underlying features of their condition [8]. The increasing prevalence of allergic disorders could be explained if they were prevented by microbial colonization in early childhood [9]. The so called hygiene hypothesis suggests that the allergy protective effect may be mediated by microbial agents associated with the presence of older siblings. In other words, infections may have a protective effect on the development of allergic disorders [9].
H. pylori, has been demonstrated worldwide and in individuals of all ages. It is estimated that 50 percent of the world’s population is affected. Infection is more frequent and acquired at an earlier age in developing countries compared with industrialized nations [10]. Once acquired, infection persists and may or may not produce gastro-duodenal disease. In developing nations, where the majority of children are infected before the age of 10, the prevalence in adults peaks at more than 80 percent before age 50 [10,11]. In developed countries, such as the United States, evidence of infection in children is unusual but becomes more common during adulthood [11]. H. pylori is a spiral shaped, microaerophilic, gram negative bacterium, the organism can be biochemically characterized as catalase, oxidase, and urease positive. The organism’s urease, motility, and ability to adhere to gastric epithelium are factors that allow it to survive and proliferate in the gastric milieu [12]. Disruption of urease activity, bacterial mobility, or attachment prevents H. pylori colonization [13]. H. pylori then attaches to gastric epithelial cells by means of specific receptor-mediated adhesion [13,14]. Although attachment is dependent upon binding of bacterial surface adhesins to specific epithelial cell receptors, host factors can modulate this process. As an example, certain individuals may express specific surface receptors or greater numbers of receptors, making them more susceptible to H. pylori attachment and colonization [15]. As H. pylori is a gram-negative bacteria; therefore, H pylori-derived LPS is considered a direct stimulator of Toll-like Receptor (TLR4)-mediated innate immunity. Previous immuno-histochemical studies revealed that the expression of TLR4 in H. pylori gastritis was higher than that in uninfected gastric mucosa [16]. Recent studies have demonstrated that TLR signals can influence intestinal homeostasis [17]. Mucosal cells and consequent activation of signaling cascades can enhance the production of pro-inflammatory cytokines and antimicrobial peptides, as well as the maintenance of the epithelial barrier function. Hence, parasitic infection can maintain the epithelial barrier function and epithelial cell proliferation through TLR signaling pathways [18].
Approval of this study was received from the administrations of the Hospitals in which the study was conducted in. The routine consents for Laboratory diagnosis were implemented for all cases according to Hospital regulations, and the study protocol conforms to the ethical Guidelines of the 1975 Declaration of Helsinki. Children were recruited through primary care physician offices and Clinics and Nor-khan General Hospital (Hafer Al Batin, Saudi Arabia) as well as Al Azhar University Hospitals Cairo, Egypt. All participants between 2 months to 7 years were investigated for H pylori using two laboratory tests.
Exclusion criteria included cases of hematologic disorders, metabolic disorders; food allergy, Celiac Disease, collagen vascular diseases, severe illness or children on antibiotics for four weeks ago or on antisecretory /steroid therapy for two weeks ago were all excluded from this study. All cases were relatively matched with the eligible control group for age, sex, race, maternal age, family size, education, and other socio-demographic variables to rule out any possible confounders.
Inclusion Criteria included cases with presumed atopic dermatitis from 2 months to 7 years old, they should have their diagnosis based on the criteria summarized in Box 1 and according to the American Academy of Dermatology [19].

Features to be considered in the diagnosis of atopic dermatitis. ESSENTIAL FEATURES-Must be present: *Pruritus *Eczema
(acute, subacute, chronic), Typical morphology and age-specific patterns , Chronic or relapsing history.
Patterns include: 1. Facial, neck, and extensor involvement in infants and children, 2. Current or previous flexural lesions in any age group, 3. Sparing of the groin and axillary regions. IMPORTANT FEATURES. Seen in most cases, adding support to the diagnosis: *Early age of onset *Atopy (Personal and/or family history , Immunoglobulin E reactivity) *Xerosis

Detection of H. pylori infection
The case and control groups were investigated for H pylori using a stool antigen test. This one-step test is a chromatographic Immune assay for the qualitative detection of H pylori infections (Alcon Laboratories Inc.). It is a relatively simple, reliable, more applicable, and noninvasive test of H pylori infectionsin children. Helicobacter pylori fecal antigen has showna high degree of sensitivity, specificity, and positive and negative predictive values [20].
Anti-H pylori antibodies test
Individuals infected with H. pylori develop antibodies that significantly correlate with the histologically confirmed cases. It is noteworthy that the geographic distributions of eligible participating children add more strength to the design of the study. There is a good correlation between ELISA antibody test and rapid urease test, which afford confirmatory diagnosis of H. pylori infection [21,22]. In this study, serum samples were assessed through ELISA for the presence of anti-H. pylori IgG antibodies against high molecular weight cell–associated protein (HM-CAP) of H. pylori using the HM-CAP ELISA kit (EZ-EM Inc. Westbury, NY, USA) as described previously [23]. All analyses were performed using SPSS (SPSS Inc.). The demographic characteristics of cases and controls were compared using the Fisher exact test, and odds Ratio.
Among the 250 enrolled participants, there was homogeneous distribution in both groups (atopic cases and non-atopic control) regarding to age, sex, race, family size, insurance status, and maternal education. In atopic cases, there were 100 girls (58.8%) and 70 boys (41.2 %). Compared to control, the enrolled girls and boys were 60% and 40% respectively. The mean age was (3.7± 1.1) and (3.5 ± 1.9) years for case and control groups respectively. Age distribution of atopic cases showed 76 cases (44.7%) in the age group (2 months to 2 years) and 94 cases (55.3 %) in age group (2 to 7 years); In control 36 (45.0%) in the age group (2 months to 2 years) and 44 (55.0%) in the age group (2 to 7 years) as in Table 1. Of the total 170 Atopic cases, 6 cases (3.5%) tested positive serologic ELISA assay and 12 cases (7.0%) tested positive H pylori Stool antigen. In control, there were 12 cases (15.0%), 6cases (7.5%) and 2cases (2.5%) tested positive by serologic ELISA, Stool antigen test and both tests respectively Table 2. For serologic testing, Odds Ratio (OR) = 0.2073, 95% Confidence Interval (CI) = 0.0748-0.5749. Z statistics = 3.024, P value = 0.0025 i.e. Significant inverse correlation between atopic dermatitis and positive H pylori serologic testing. For H pylori stool antigen testing, OR = 0.9367, 95% CI = 0.3384 to 2.5929, Z statistics = 0.126, P value = 0.8998, No significant correlation between atopic dermatitis and positive H pylori stool antigen Table 3.
Eligible cases were enrolled from outpatient clinics of Al-Azhar University Hospitals (Cairo, Egypt) and from Nor-Khan General Hospital (Saudi Arabia). Informed consents were obtained from Parents or Guardians according to hospital regulations. Printed records were collected to verify inclusion criteria, medications given, clinical review, family and past histories. Cases with presumed atopic dermatitis from 2months to 7years old should have their diagnosis based on the criteria summarized in Box 1 and according to the American Academy of Dermatology. There was homogeneous distribution in both groups (atopic cases and non-atopic control) regarding to age, sex, race, family size, insurance status, and maternal education.
Characteristic* Atopic cases (N = 170) Control (N = 80)
2 Months-2 yrs. 76 (44.7%) 36 (45.0%)
2 Yrs-7 yrs. 94 (55.3%) 44 (55.0%)
Mean Age, yrs. (3.7 ± 1.1) (3.5 ± 1.9)
Female 100 (58.8%) 48 (60.0%)
Male 70 (41.2%) 32 (40.0%)
Child Nationality
Egyptian 64 (37.6%) 32 (40.0%)
Saudian 58 (34.1%) 28 (35.0%)
Others 48 (28.2%) 20 (25.0%)
*Socioeconomic status is based on parental occupation. Education was nearly balanced among participant’sparents, particularlywhen considering the average level nature of the hospital community.
Table 1: Demographic characteristics of 250 participants including case group (Atopic patients) and Control group.
Test Atopic Cases (N = 170) Control (N = 80)
+ELISA 6 (3.5%) 12 (15.0%)
+H pylori stool antigen 12 (7.0%) 6 (7.5%)
+Both -- 2 (2.5%)
Total 18 20
Table 2: Laboratory testing of Helicobacter pylori(H pylori) in Atopic Cases versus Control.
Parameter ELISA Test H pylori stool antigen Test
Odds ratio 0.2073 0.9367
95% CI: 0.0748 to 0.5749 0.3384 to 2.5929
z statistic 3.024 0.126
Significance level P = 0.0025 P = 0.8998
Table 3:Statistical Comparison between ELISA and H pylori stool antigen testing for the studied groups.
The results of this study suggest that the relationship between childhood eczema and H pylori infection is a complex one, at least in this clinical sample of children referred for H pylori testing. The genetic diversity of H. pylori and the variations in human host response to the microorganism underlie the complex host-pathogen relationship that determines the natural history of infection. Since the relationship between infections and eczema is not a simple one, it is not surprising that some studies confirmed the inverse association between atopy/allergic diseases and H pylori infection. Other studies claimed that the association is causal and directly proportional. According to the hygiene hypothesis [24] when children are brought up exposed to allergens in the environment at a young age, their immune system is more likely to tolerate them, while children brought up in a modern “sanitary” environment are less likely to be exposed to those allergens at a young age, andwhen they are finally exposed, develop allergies. There is some support for this hypothesis with respect to AD.
A meta-analysis reported a favorable effect of exposure to dogs and pets on the risk of AD in infants or children, whereas no association emerged with exposure to cats [24]. To our knowledge, our study is the first to clearly evaluate the potential role of H pylori infection in Childhood atopic dermatitis in developing countries. Moreover, the study may disclose the apparent ambiguity related to the contradiction between some clinical studies that confirmed an inverse association between H. pylori and atopy, and other studies that reported an antagonistic result [25]. A recent study have indicated that differences in gut microbiota do precede development of atopy [26] and early postnatal or even pre-natal factors, such as an alteration of the gut micro flora, play an important role in eczema development [27]. The more recent research conducted by Zhou., et al. demonstrated the role of lactobacilli in treating H pylori–related diseases, and the results indicated that viable lactobacilli prevented the development of H pylori Sydney strain 1 (SS1) lipo polysaccharide (LPS)–activated Toll-like receptor (TLR4) pathways in SGC-7901 cells, leading to the inhibitory effects of lactobacilli on IL-8 production stimulated by H pylori SS1 LPSs [28]. Schmidt., et al. demonstrated that TLR4 is involved in the development of contact allergy to nickel in humans and was attributed toactivation of the pro inflammatory intracellular signal transduction cascades [29]. Other data implicate site-specific human TLR4 inhibition as a potential strategy for therapeutic intervention in contact hypersensitivity that would not affect vital immune responses [29]. Toll-like receptors have also been shown to be an important link between innate and adaptive immunity through their presence in dendritic cells. The TLRs 3 and 4 are present on the surface of monocyte derived dendritic cells. TLRs have also been shown to be expressed on immune cells like T cells [30]. It is reported that low level LPS signaling through TLR4 is necessary to induce Th2 responses. The mechanism by which LPS signaling results in Th2 sensitization involves the activation of antigen-containing dendritic cells. In contrast to low levels, high levels of LPS with antigen resultedin Th1 responses. These studies suggested that the level of LPS exposure can determine the type of inflammatory response generated [31]. Other results demonstrated the crucial and innate role of TLR4 in promoting the activation of CD4+ and γδ T cells, which contributes to the initiation of autoimmune inflammation [32]. Other studies clearly reported that H pylori seropositivity was highly prevalent in rural Tanzania and Seropositive persons showed a Th2-dominant immune response to H pylori infection, which may be due to effects of concurrent infection with parasites and/or bacterial infections [33]. Taken together, it is evident that Toll-like receptor signaling pathway activation mediated the H pylori immunologic aspect of pathogenesis. Our results reported a significant inverse relation between atopic dermatitis and positive Serology for H pylori infection with its implication of definite enhancement of TLR4 signaling cascade central to production of pro- inflammatory cytokines that ultimately direct the activation of Th2---› B cell ---› IGE atopic response instead of Th2---› B cell ---› IGg response, normally encountered in non-atopic population. LPS, also termed endotoxin, represents the main surface antigen (O-antigen) for Gram-negative bacteria. Itwas released when the bacteria underwent lysis [34]. Accordingly, LPS concentration is directly proportional to the degree of H pylori cell lysis. Thus, induction of TLR-Th2-B cell atopic pathway is evidently caused by the persistent low level of bacterial cell death. This probably could elucidate the slowly progressive release of pro- inflammatory cytokines and antimicrobial peptides as well as the maintenance of the epithelial barrier function and epithelial cell proliferation characteristic of TLR signaling pathways. This pattern of adaptive immunologic pathway expressed in gut milieu could not mount an anti-H pylori IGg antibodies. On the contrary, the non-significant association of atopic dermatitis with H pylori stool antigen test may be explained by the observation that high levels of LPS implicated high antigen load with the resultant Th1 responses (cell mediated non atopic response). As the high levels of LPS indicate instant rapid H pylori lysis, this means that H pylori is readily attacked by an antimicrobial factor in the gut, which may be another micro biota flora, helminthes or its product, or even antibiotics. This may uncover and explain the conclusions of the following studies:
1 The role of Lactobacillus reuteri in reducing atopic eczema in childhood [35].
2 Epidemiological studies reported a protective role for Helminthes against AD [36].
3 Helicobacter, the germ that causes stomach ulcers can also trigger eczema [37].
4 Corrado., et al demonstrated a positive association between H pylori infection and food allergies in thirty children who were suf- fering digestive symptoms [38].
5 Galadari., et al reported that the incidence of H pylori in 20 atopic dermatitis patients was considerably higher than that of control subjects [39].
During pregnancy, the cytokine inflammatory-response profile of the fetus is diverted away from cell-mediated immunity (T-helper 1) toward humeral immunity (i.e) Th2 type. Hence, the Th2 type is typically the general immune response in early infancy. The risk of allergic disease could will be the result of a lack or delay in the eventual shift of the predominant Th2 type of response to more of a balance between Th1- and Th2-type responses [40]. Administration of probiotic bacteria during the time, in which a natural population of lactic-acid–producing intestinal bacteria is developing, could theoretically influence immune development toward more balance of Th1 and Th2 inflammatory responses [41]. The intestinal bacterial flora of atopic children has been demonstrated to differ from that of non-atopic children [42,43] which has served as rationale for theadministration of probiotics to infants at risk of atopic diseases, particularly for those who are formula fed.
In short
The apparent inverse relation between atopic dermatitis and H pylori seropositivity could be expected in atopic infants as atopic infants divert the Th2 type(B cell --›IGg) response of H pylori to Th2 type (B cell --›IGE) response. As the infant grows up, eventual shift of the predominant Th2 type of response to more of a balance between Th1- and Th2-type responses. The insignificant relation between atopic dermatitis and H pylori stool antigen testing (H pylori colonization) could be explained as follows; H pylori colonization does not necessarily invoke inflammatory immune responses as evidenced by the well documented asymptomatic and subclinical cases of H pylori infections. Moreover, colonization is instantly opposed and antagonized by multiple factors including; competitive inhibition by another gut microbiota or Helminthes or its products, host factors (genetic susceptibility, diet) environmental, geographic variation, and wide usage of antibiotics. All these factors imply disproportionate correlation between colonization and the atopic immunologic response to H pylori infection.
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Copyright: © 2015 Abdelrazak ali., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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EC Ophthalmology
OCT-Angiography for Non-Invasive Monitoring of Neuronal and Vascular Structure in Mouse Retina: Implication for Characterization of Retinal Neurovascular Coupling

PMID: 29333536 [PubMed]

PMCID: PMC5766278

EC Neurology
Longer Duration of Downslope Treadmill Walking Induces Depression of H-Reflexes Measured during Standing and Walking.

PMID: 31032493 [PubMed]

PMCID: PMC6483108

EC Microbiology
Onchocerciasis in Mozambique: An Unknown Condition for Health Professionals.

PMID: 30957099 [PubMed]

PMCID: PMC6448571

EC Nutrition
Food Insecurity among Households with and without Podoconiosis in East and West Gojjam, Ethiopia.

PMID: 30101228 [PubMed]

PMCID: PMC6086333

EC Ophthalmology
REVIEW. +2 to +3 D. Reading Glasses to Prevent Myopia.

PMID: 31080964 [PubMed]

PMCID: PMC6508883

EC Gynaecology
Biomechanical Mapping of the Female Pelvic Floor: Uterine Prolapse Versus Normal Conditions.

PMID: 31093608 [PubMed]

PMCID: PMC6513001

EC Dental Science
Fiber-Reinforced Composites: A Breakthrough in Practical Clinical Applications with Advanced Wear Resistance for Dental Materials.

PMID: 31552397 [PubMed]

PMCID: PMC6758937

EC Microbiology
Neurocysticercosis in Child Bearing Women: An Overlooked Condition in Mozambique and a Potentially Missed Diagnosis in Women Presenting with Eclampsia.

PMID: 31681909 [PubMed]

PMCID: PMC6824723

EC Microbiology
Molecular Detection of Leptospira spp. in Rodents Trapped in the Mozambique Island City, Nampula Province, Mozambique.

PMID: 31681910 [PubMed]

PMCID: PMC6824726

EC Neurology
Endoplasmic Reticulum-Mitochondrial Cross-Talk in Neurodegenerative and Eye Diseases.

PMID: 31528859 [PubMed]

PMCID: PMC6746603

EC Psychology and Psychiatry
Can Chronic Consumption of Caffeine by Increasing D2/D3 Receptors Offer Benefit to Carriers of the DRD2 A1 Allele in Cocaine Abuse?

PMID: 31276119 [PubMed]

PMCID: PMC6604646

EC Anaesthesia
Real Time Locating Systems and sustainability of Perioperative Efficiency of Anesthesiologists.

PMID: 31406965 [PubMed]

PMCID: PMC6690616

EC Pharmacology and Toxicology
A Pilot STEM Curriculum Designed to Teach High School Students Concepts in Biochemical Engineering and Pharmacology.

PMID: 31517314 [PubMed]

PMCID: PMC6741290

EC Pharmacology and Toxicology
Toxic Mechanisms Underlying Motor Activity Changes Induced by a Mixture of Lead, Arsenic and Manganese.

PMID: 31633124 [PubMed]

PMCID: PMC6800226

EC Neurology
Research Volunteers' Attitudes Toward Chronic Fatigue Syndrome and Myalgic Encephalomyelitis.

PMID: 29662969 [PubMed]

PMCID: PMC5898812

EC Pharmacology and Toxicology
Hyperbaric Oxygen Therapy for Alzheimer's Disease.

PMID: 30215058 [PubMed]

PMCID: PMC6133268

News and Events

December Issue Release

We always feel pleasure to share our updates with you all. Here, notifying you that we have successfully released the November issue of respective journals and the latest articles can be viewed on the current issue pages.

Submission Deadline for Upcoming Issue

ECronicon delightfully welcomes all the authors around the globe for effective collaboration with an article submission for the upcoming issue of respective journals. Submissions are accepted on/before December 20, 2022.

Certificate of Publication

ECronicon honors with a "Publication Certificate" to the corresponding author by including the names of co-authors as a token of appreciation for publishing the work with our respective journals.

Best Article of the Issue

Editors of respective journals will always be very much interested in electing one Best Article after each issue release. The authors of the selected article will be honored with a "Best Article of the Issue" certificate.

Certifying for Review

ECronicon certifies the Editors for their first review done towards the assigned article of the respective journals.

Latest Articles

The latest articles will be updated immediately on the articles in press page of the respective journals.