
2Graduate School of Natural Science and Technology, Okayama University, Japan
3Department of Soil Science, Bangladesh Agricultural University, Bangladesh
The pot experiments were carried out at Net-house of the Department of Soil Science, Bangladesh Agricultural University (BAU), and Mymensingh. Equal size plastic pots were prepared with 8 kg soils. Characteristically, the soil was silt loam having pH 6.15, electrical conductivity 0.17 dS/m, exchangeable Na 0.35 meq/100g soil, total N 0.11% and organic matter 1.90%. Two high yielding rice (Oryza sativa L.) cultivars viz. BRRI dhan29 (salt-sensitive) and Binadhan-8 (moderately salt-tolerant) were used as plant materials. Nine treatment combinations viz. control (no NaCl or proline), 25 mM NaCl, 25 mM NaCl + 25 mM proline, 25 mM NaCl + 50 mM proline, 25 mM NaCl + 100 mM proline, 50 mM NaCl, 50 mM NaCl + 25 mM proline, 50 mM NaCl + 50 mM proline, 50 mM NaCl + 100 mM proline were used for the two rice cultivars. Rice seedlings were grown in non-saline silt loam soils. Three healthy seedlings of thirty-day-old were transplanted in each hill of each pot. The pure salt (NaCl) was used for developing salinity. Rice plants were exposed to different concentrations of NaCl at 30 days after transplanting (vegetative stage). On the same day, different doses of proline containing 0.1% Tween-20 were sprayed on the leaves at a volume of 25 mL per plant as per treatment. Similarly, proline was applied at 65 days after transplanting (panicle initiation stage) as per treatment. The experiment was laid out in a completely randomized design with four replications.
Normal water was used as irrigation. Fertilization and other management practices were performed as and when required. At 45 days after transplanting (15 days after first proline application), healthy green leaves were detached from the plants for the determination of chlorophyll, proline and ascorbate contents, and activity of antioxidant enzymes such as CAT, POX and APX. The crop was harvested at full maturity. Grain and straw yields and plant parameters were recorded. K and Na contents were measured from grain and straw samples.
Chlorophyll content was measured according to Porra., et al. [29]. An aliquot amount of fresh green leaf was suspended in 10 mL of 80% acetone, mixed well and kept at room temperature in the dark for 7 days. The supernatant was collected after centrifugation at 5000 rpm for 15 min. After that, the absorbance was recorded at 645 nm and 663 nm using a spectrophotometer.
Proline content was measured according to the method of Bates., et al. [30]. An aliquot amount of fresh green leaf was homogenized in 10 mL of 3% sulfosalicylic acid and the homogenate was centrifuged at 5000 rpm for 15 min. Two milliliters of the supernatant were reacted with 2 mL of acid ninhydrin (1.25 g ninhydrin dissolved in 30 mL of glacial acetic acid and 20 mL of 6 M phosphoric acid) and 2 mL of glacial acetic acid for 1 hr at 100°C and the reaction was then terminated in an ice bath. The colored reaction mixture was extracted with 4 mL of toluene and the absorbance was recorded at 520 nm. Proline content was calculated from a standard curve.
Ascorbate content was determined by 2,6-dichlorophenolindophenol visual titration method where ascorbate stoichiometrically reduces the dye 2,6-dichlorophenolindophenol to colorless compound. A quantity of 0.5 g green leaf with 10 mL of 3% of metaphosphoric acid solution was blended in a blender to yield homogenous extract. The whole extract was then filtered through a piece of cheese cloth and washed with 3% metaphosphoric acid solution. Ten milliliters of aliquot of the filtrate in triplicate were titrated against the standardized dye.
An aliquot amount of fresh green leaf was homogenized with 5 mL of 50 mM Tris-HCl buffer (pH 8.0) for CAT, and 50 mM KH2PO4 buffer (pH 7.0) for POX and APX. The homogenate was centrifuged at 5000 rpm for 20 min and the supernatant was then used as enzyme extract.
CAT (EC: 1.11.1.6) activity was determined according to the method of Aebi [31]. The reaction mixture consisted of 50 mM Tris-HCl buffer (pH 8.0), 0.25 mM EDTA, 20 mM H2O2 and 25 µL of enzyme extract. The reaction was started by the addition of H2O2. The activity was calculated from the decrease in absorbance at 240 nm for 2 min when the extinction coefficient was 40 mM-1 cm-1. POX (EC: 1.11.1.7) activity was determined according to Nakano and Asada (1981). The reaction buffer solution contained 50 mM KH2O4 buffer (pH 7.0), 0.1 mM EDTA, 0.1 mM H2O2, and 10 mM guaiacol. The reaction was started by adding enzyme extract to the reaction buffer solution. The activity was calculated from change in absorbance at 470 nm for 30 sec where an extinction coefficient is 26.6 mM-1 cm-1. APX (EC: 1.11.1.11) activity was measured following the method of Nakano and Asada [32]. The reaction buffer solution contained 50 mM KH2O4 buffer (pH 7.0), 0.1 mM EDTA, 0.1 mM H2O2, and 0.5 mM ascorbate. The reaction was started by the addition of enzyme extract to the reaction buffer solution. The activity was calculated from the change in absorbance at 290 nm for 1 min when the extinction coefficient was 2.8 mM-1 cm-1.
Grain and straw samples were dried in an oven at about 65°C for 48 hours and then ground in a grinding machine to pass through a 20 mesh sieve. Grinding samples of 0.5 g (grain and straw, separately) were transferred into 100 mL digestion vessel. Ten milliliters of diacid mixture (HNO3:HClO4 = 2:1) were added into the vessel. After leaving for a while, the flasks were heated at a temperature slowly raised to 200°C. Heating was stopped when the dense white fume of HClO4 occurred. After cooling, the content was taken into a 50 mL volumetric flask and the volume was made with distilled water. This digest was used for the determination of K and Na.
Five milliliters digest for grain and 2 mL digest for straw were taken, and both digests are diluted to make a desired concentration. Then K and Na contents were determined using a flame photometer according to Brown and Lilleland [33].
Data were analyzed statistically using analysis of variance with the help of software package of MSTAT-C. The significant differences between mean values were compared by Duncan’s Multiple Range Test. Differences at P < 0.05 were considered significant.
Salt stress caused a significant reduction in growth and yield of salt-sensitive (BRRI dhan29) and salt-tolerant (Binadhan-8) rice (Table 1). NaCl stress at 50 mM caused a drastic reduction in growth parameters of both rice cultivars. Neither salt-sensitive nor salt-tolerant rice produced effective tillers as well as grains after exposure to 50 mM NaCl stress. It was also observed that all plants of both cultivars died within 15 days after exposure to 50 mM NaCl stress. Foliar application of proline (25-100 mM) resulted in an increase in growth, yield contributing characters and yield of both rice cultivars in response to 25 mM NaCl stress. It is important to note that salt-tolerant (Binadhan-8) rice cultivar produced effective tillers and grains at 50 mM NaCl with 100 mM proline application, although salt-sensitive rice cultivar (BRRI dhan29) failed to do so (Table 1).
Treatments | BRRI dhan29 | Binadhan-8 | ||||||
Plant dry weight (g/pot) | No. of effective tillers /hill | No. of filled grains/ panicle | Grain weight (g/pot) | Plant dry weight (g/pot) | No. of effective tillers /hill | No. of filled grains/ panicle | Grain weight (g/pot) | |
T0: Control | 49.3a | 20a | 141a | 45.3a | 57.1a | 22a | 135a | 45.0a |
T1: 25 mM NaCl | 15.0c | 9d | 119c | 20.1e | 15.5d | 8d | 81e | 16.5d |
T2: 25 mM NaCl + 25 mM proline | 32.5b | 13c | 139a | 36.9b | 24.2c | 13b | 99d | 27.4c |
T3: 25 mM NaCl + 50 mM proline | 33.8b | 15b | 126b | 32.8d | 28.1b | 12c | 120b | 32.5b |
T4: 25 mM NaCl + 100 mM proline | 32.4b | 13c | 139a | 34.5c | 28.2b | 13b | 105c | 29.6c |
T5: 50 mM NaCl | 4.51d | ND | ND | ND | 2.79f | ND | ND | ND |
T6: 50 mM NaCl + 25 mM proline | 4.65d | ND | ND | ND | 4.50e | ND | ND | ND |
T7: 50 mM NaCl + 50 mM proline | 4.69d | ND | ND | ND | 3.58ef | ND | ND | ND |
T8: 50 mM NaCl + 100 mM proline | 5.58d | ND | ND | ND | 14.8d | 6e | 59f | 12.9e |
SE (±) | 5.65 | 2.63 | 23.44 | 6.33 | 5.75 | 2.52 | 18.11 | 5.48 |
CV% | 4.92 | 4.31 | 2.62 | 4.65 | 3.87 | 4.06 | 3.17 | 8.65 |
Chlorophyll a content was significantly decreased in salt-tolerant rice in response to salt stress, although chlorophyll b and total chlorophyll contents were significantly lower in both salt-sensitive and salt-tolerant rice cultivars during salt stress than non-stress (Table 2). On the contrary, foliar application of proline showed significant increases in chlorophyll a, b and total chlorophyll contents in both rice cultivars with 25 mM NaCl stress condition. At 50 mM NaCl stress, exogenous proline also offered a significant amount of chlorophyll contents in salt-tolerant rice (Table 2).
Treatments | BRRI dhan29 | Binadhan-8 | ||||
Chl-a (µg/ml) | Chl-b (µg/ml) | Total Chl (µg/ml) | Chl-a (µg/ml) | Chl-b (µg/ml) | Total Chl (µg/ml) | |
T0: Control | 5.51d | 9.98a | 15.5a | 5.40b | 8.98c | 14.4b |
T1: 25 mM NaCl | 5.66d | 7.20c | 12.9b | 4.96c | 6.46e | 11.4c |
T2: 25 mM NaCl + 25 mM proline | 6.66b | 8.42b | 15.1a | 5.45b | 9.31bc | 14.7ab |
T3: 25 mM NaCl + 50 mM proline | 6.97a | 8.32b | 15.4a | 5.58b | 9.88a | 15.5a |
T4: 25 mM NaCl + 100 mM proline | 6.25c | 8.19b | 14.4a | 6.89a | 8.19d | 15.1ab |
T5: 50 mM NaCl | ND | ND | ND | ND | ND | ND |
T6: 50 mM NaCl + 25 mM proline | ND | ND | ND | ND | ND | ND |
T7: 50 mM NaCl + 50 mM proline | ND | ND | ND | ND | ND | ND |
T8: 50 mM NaCl + 100 mM proline | ND | ND | ND | 5.67b | 9.57ab | 15.2ab |
SE (±) | 1.10 | 1.50 | 2.59 | 0.96 | 1.49 | 2.43 |
CV% | 4.60 | 6.19 | 10.21 | 6.55 | 4.79 | 6.84 |
Salt stress significantly increased intracellular proline levels in salt-sensitive rice but decreased in salt-tolerant rice (Figure 1A). Exogenous proline showed a significant increase in proline accumulation in both rice varieties at 25 mM NaCl stress. The proline accumulation was much higher in salt-tolerant rice than salt-sensitive when 50 or 100 mM proline was exogenously applied at NaCl-stressed plants. Additionally, the intracellular proline level was higher in plants treated with 50 mM NaCl + 100 mM proline than 25 mM NaCl (Figure 1A).
Ascorbate contents were measured in rice to investigate whether exogenous proline influenced antioxidant defense system (Figure 1B). Ascorbate contents in salt-sensitive rice was not affected by salt stress, but significantly increased in salt-tolerant rice. Exogenous application of proline showed a significant increase in ascorbate contents in salt-sensitive rice at 25 mM NaCl stress, however, no increase of ascorbate contents was observed in salt-tolerant rice. On the contrary, 100 mM proline resulted in a significant increase in ascorbate content in salt-tolerant rice responses to 50 mM NaCl stress (Figure 1B).

T0: control (no NaCl or proline), T1: 25 mM NaCl, T2: 25 mM NaCl + 25 mM proline, T3: 25 mM NaCl + 50 mM proline, T4: 25 mM NaCl + 100 mM proline, T5: 50 mM NaCl, T6: 50 mM NaCl + 25 mM proline, T7: 50 mM NaCl + 50 mM proline, T8: 50 mM NaCl + 100 mM proline.
We investigated whether proline enhanced the activities of H2O2-scavenging antioxidant enzymes (Figure 2B-D). Salt stress caused significant reductions in CAT, POX and APX activities in salt-sensitive rice, and POX activity in salt-tolerant rice. Proline application showed significant increases in CAT, POX and APX activities of salt-sensitive rice in response to salt stress. In salt-tolerant rice, salt stress resulted in an increase in CAT activity, and this increase was more prominent in the presence of proline. It was also observed that CAT activity was significantly higher at 50 mM NaCl + 100 mM proline than non-stress. POX activities were significantly increased in salt-tolerant rice cultivar under different concentrations of proline application at 25 mM NaCl or even 50 mM NaCl stress condition. APX activity was also increased in salt-tolerant rice cultivar under salt stress, although exogenous application of proline did not show any increase in APX activity (Figure 2D).

Salt stress significantly decreased K+/Na+ ratio in both salt-sensitive and salt-tolerant rice cultivars (Figure 3A, B). Exogenous proline increased straw K+/Na+ ratio in both cultivars but these increases were not consistent. The straw K+/Na+ ratio was also high in salt-tolerant rice when exposed to 50 mM NaCl with 100 mM proline (Figure 3A). The grain K+/Na+ ratio was higher in salt-tolerant rice than salt-sensitive rice even exposure to NaCl and proline (Figure 3B). However, exogenous proline resulted in a significant increase in grain K+/Na+ ratio in both cultivars (Figure 3B).

We investigated the changes in soil properties such as pH, EC, exchangeable Na, organic matter and total N of post-harvest soils (Table 3). A considerable increase in soil pH, EC and exchangeable Na was observed under NaCl stress. No remarkable changes in soil organic matter and total N were observed due to NaCl stress. Foliar proline treatments on rice cultivars had no influence on soil properties under salt stress conditions (Table 2).
In the present study, we investigated the protective effects of exogenous proline on rice against NaCl-induced damage. Previously we have shown that exogenous proline improves cell growth and suppresses cell death in tobacco cultured cells induced by salt stress [7,13,14,23,24,]. Sobahan., et al. [28] have shown that exogenous proline improves salt tolerance in salt-sensitive rice more effectively than salt-tolerant rice. In this experiment, exogenous proline increased growth and yield of both salt-sensitive and salt-tolerant rice at 25 mM NaCl stress (Table 1). Further, salt-tolerant rice plants survived and produced effective tillers and grains in response to 50 mM NaCl stress in the presence of 100 mM proline. In contrast, none of plants of both cultivars survived in response to 50 mM NaCl stress even application of 25-100 mM proline (Table 1). There are increasing evidences that exogenously supplied proline improves growth of a variety of plant species in response to salt stress [9,12,26,27,34]. These results suggest that the adverse effects of salt stress on plants could be alleviated by application of proline. These results also suggest that salt-tolerant plant over expressing proline accumulation could contribute to the improvement of salinity tolerance.
Chlorophyll is one of the most important pigment components of a plant. Measurement of chlorophyll content provides the quantitative information about photosynthesis. Chlorophyll content may vary due to varying salt stress levels, affecting plant growth and development. The reduction in plant growth in the present investigation subjected to salinity is associated with a decreased rate of photosynthetic capacity. Chlorophyll contents in both salt-sensitive and salt-tolerant rice decreased due to salt stress whereas this decrease was alleviated by exogenous application of proline (Table 2). Similarly, there are evidences that salinity leads to a decrease in chlorophyll contents in rice genotypes [28,35,36]. Some authors have also shown that exogenous proline reduces the adverse effects of salt stress by increasing photosynthetic activity and chlorophyll contents in a variety of plants including rice [28,34,37], indicating that improved plant growth of rice cultivars due to exogenous proline under salt stress might be positively correlated with an increased rate of photosynthetic capacity.
Increased levels of proline accumulated in plants correlate with improved salt tolerance [7-9]. Exogenous application of proline remarkably increases proline accumulation under NaCl stress and mitigates NaCl-induced growth inhibition [7,23], indicating that uptake of compatible solutes plays an important role in adaptation to osmotic stress caused by salinity. A significant increase in intracellular proline content was observed in salt-sensitive rice but not in salt-tolerant rice responses to salt stress (Figure 1B). Exogenous proline increased the intracellular proline levels in both rice cultivars which were positively associated with the improvement of salt tolerance (Figure 1B). Similar to our results, Summart., et al. [38] and Nounjan., et al. [9] showed that salt stress caused an increase in proline accumulation in rice. Nounjan., et al. [9] also showed that exogenous application of proline increased endogenous proline in rice plants. The concentration of proline, however, is not high enough to adjust the osmotic potential in some plants under salt stress [39]. Proline has been suggested to function as an antioxidant in protecting cells against the damaging effects of various stresses since proline scavenges free radicals and suppresses ROS accumulation [4,7,10,13,14,25].
Up-regulation of the antioxidant defense mechanisms correlates with the alleviation of oxidative damage and improved tolerance to salinity [4,11,20-24]. Plants employ both enzymatic and non-enzymatic antioxidant defense systems against NaCl-induced oxidative damage. This defense system is impaired due to salt stress. On the contrary, proline enhances antioxidant defense mechanisms against NaCl-induced damage and improves salt tolerance in various plants as well as in cultured cells [7,9,11-14,23-27]. In order to elucidate the antioxidant defense mechanism of proline, we measured ascorbate content and activities of major H2O2-scavenging antioxidant enzymes CAT, POX and APX. In plant cells, ascorbate is a major antioxidant that directly scavenges ROS and act as an electron donor to APX for scavenging H2O2 involved in the ascorbate-glutathione cycle [18,19]. A significant increase in ascorbate content was observed in salt-tolerant but not in salt-sensitive rice responses to salinity (Figure 2A). The opposite results were observed in the presence of proline at 25 mM NaCl stress although 100 mM proline significantly increased ascorbate content in salt-tolerant rice at 50 mM NaCl stress (Figure 2A). Several authors have shown that salt stress leads to a decrease in ascorbate content in salt-sensitive cultivars [20-24,40,41]. An increase in ascorbate contents has been reported in tobacco cultured cells under NaCl stress in the presence of exogenous proline [24]. The biosynthetic capacity of ascorbate is impaired under stress conditions because the ascorbate pool is generally determined by its rates of not only regeneration but also synthesis [42]. However, increased ascorbate contents in proline-treated plants under salinity are probably due to its regeneration or synthesis process accelerated by proline or decreased activity of APX where ascorbate is used as a reductant.
A low ratio of Na+ to K+ in the cytosol is essential for normal cellular functions of plants. Na+ competes with K+ uptake, causing an increase in Na+ to K+ ratio in the cytosol under salt stress, resulting in accumulation of toxic levels of Na+ and insufficient K+ concentrations for enzymatic reactions and osmotic adjustment [5,45]. Exogenously supplied proline reduces Na+ accumulation and increases K+/Na+ ratio under salt stress [9,28,34]. In contrast, exogenous proline has been shown to alleviate the inhibition of NaCl-induced cell growth without improving a ratio of K+ to Na+ [7]. Salt stress significantly reduced K+/Na+ ratio in both rice cultivars whereas this K+/Na+ ratio was increased by proline application under NaCl stress (Figure 3A,B). These results indicate that exogenous proline contributes to the reduction of Na uptake as well as increment of K uptake under salt stress condition.
Saline soils have detrimental effects on soil physical and chemical properties, and cause nutrient deficiencies [46]. It was observed that salt stress considerably increased soil pH, EC and exchangeable Na, whereas no remarkable changes in organic matter and total N were observed after harvest of rice in soil (Table 3). Similar results have also been found in our previous report [47]. Under salt stress, foliar application of proline did not affect soil properties after post-harvest (Table 3).
Treatment | Soil properties | ||||
Soil pH | EC (dS m-1) (soil:water = 1:5) | Exchangeable Na (me/100g) | Organic matter (%) | Total N (%) | |
T0: Control | 6.17 | 0.180 | 0.35 | 1.88 | 0.108 |
T1: 25 mM NaCl | 6.50 | 0.583 | 0.63 | 1.84 | 0.106 |
T2: 25 mM NaCl + 25 mM proline | 6.48 | 0.582 | 0.64 | 1.84 | 0.106 |
T3: 25 mM NaCl + 50 mM proline | 6.48 | 0.580 | 0.64 | 1.85 | 0.106 |
T4: 25 mM NaCl + 100 mM proline | 6.49 | 0.582 | 0.63 | 1.85 | 0.105 |
T5: 50 mM NaCl | 6.62 | 1.971 | 1.28 | 1.82 | 0.104 |
T6: 50 mM NaCl + 25 mM proline | 6.61 | 1.970 | 1.27 | 1.83 | 0.104 |
T7: 50 mM NaCl + 50 mM proline | 6.61 | 1.968 | 1.28 | 1.83 | 0.104 |
T8: 50 mM NaCl + 100 mM proline | 6.62 | 1.970 | 1.28 | 1.83 | 0.105 |
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PMCID: PMC6155992
EC Orthopaedics
Distraction Implantation. A New Technique in Total Joint Arthroplasty and Direct Skeletal Attachment.
PMID: 30198026 [PubMed]
PMCID: PMC6124505
EC Pulmonology and Respiratory Medicine
Prevalence and factors associated with self-reported chronic obstructive pulmonary disease among adults aged 40-79: the National Health and Nutrition Examination Survey (NHANES) 2007-2012.
PMID: 30294723 [PubMed]
PMCID: PMC6169793
EC Dental Science
Important Dental Fiber-Reinforced Composite Molding Compound Breakthroughs
PMID: 29285526 [PubMed]
PMCID: PMC5743211
EC Microbiology
Prevalence of Intestinal Parasites Among HIV Infected and HIV Uninfected Patients Treated at the 1o De Maio Health Centre in Maputo, Mozambique
PMID: 29911204 [PubMed]
PMCID: PMC5999047
EC Microbiology
Macrophages and the Viral Dissemination Super Highway
PMID: 26949751 [PubMed]
PMCID: PMC4774560
EC Microbiology
The Microbiome, Antibiotics, and Health of the Pediatric Population.
PMID: 27390782 [PubMed]
PMCID: PMC4933318
EC Microbiology
Reactive Oxygen Species in HIV Infection
PMID: 28580453 [PubMed]
PMCID: PMC5450819
EC Microbiology
A Review of the CD4 T Cell Contribution to Lung Infection, Inflammation and Repair with a Focus on Wheeze and Asthma in the Pediatric Population
PMID: 26280024 [PubMed]
PMCID: PMC4533840
EC Neurology
Identifying Key Symptoms Differentiating Myalgic Encephalomyelitis and Chronic Fatigue Syndrome from Multiple Sclerosis
PMID: 28066845 [PubMed]
PMCID: PMC5214344
EC Pharmacology and Toxicology
Paradigm Shift is the Normal State of Pharmacology
PMID: 28936490 [PubMed]
PMCID: PMC5604476
EC Neurology
Examining those Meeting IOM Criteria Versus IOM Plus Fibromyalgia
PMID: 28713879 [PubMed]
PMCID: PMC5510658
EC Neurology
Unilateral Frontosphenoid Craniosynostosis: Case Report and a Review of the Literature
PMID: 28133641 [PubMed]
PMCID: PMC5267489
EC Ophthalmology
OCT-Angiography for Non-Invasive Monitoring of Neuronal and Vascular Structure in Mouse Retina: Implication for Characterization of Retinal Neurovascular Coupling
PMID: 29333536 [PubMed]
PMCID: PMC5766278
EC Neurology
Longer Duration of Downslope Treadmill Walking Induces Depression of H-Reflexes Measured during Standing and Walking.
PMID: 31032493 [PubMed]
PMCID: PMC6483108
EC Microbiology
Onchocerciasis in Mozambique: An Unknown Condition for Health Professionals.
PMID: 30957099 [PubMed]
PMCID: PMC6448571
EC Nutrition
Food Insecurity among Households with and without Podoconiosis in East and West Gojjam, Ethiopia.
PMID: 30101228 [PubMed]
PMCID: PMC6086333
EC Ophthalmology
REVIEW. +2 to +3 D. Reading Glasses to Prevent Myopia.
PMID: 31080964 [PubMed]
PMCID: PMC6508883
EC Gynaecology
Biomechanical Mapping of the Female Pelvic Floor: Uterine Prolapse Versus Normal Conditions.
PMID: 31093608 [PubMed]
PMCID: PMC6513001
EC Dental Science
Fiber-Reinforced Composites: A Breakthrough in Practical Clinical Applications with Advanced Wear Resistance for Dental Materials.
PMID: 31552397 [PubMed]
PMCID: PMC6758937
EC Microbiology
Neurocysticercosis in Child Bearing Women: An Overlooked Condition in Mozambique and a Potentially Missed Diagnosis in Women Presenting with Eclampsia.
PMID: 31681909 [PubMed]
PMCID: PMC6824723
EC Microbiology
Molecular Detection of Leptospira spp. in Rodents Trapped in the Mozambique Island City, Nampula Province, Mozambique.
PMID: 31681910 [PubMed]
PMCID: PMC6824726
EC Neurology
Endoplasmic Reticulum-Mitochondrial Cross-Talk in Neurodegenerative and Eye Diseases.
PMID: 31528859 [PubMed]
PMCID: PMC6746603
EC Psychology and Psychiatry
Can Chronic Consumption of Caffeine by Increasing D2/D3 Receptors Offer Benefit to Carriers of the DRD2 A1 Allele in Cocaine Abuse?
PMID: 31276119 [PubMed]
PMCID: PMC6604646
EC Anaesthesia
Real Time Locating Systems and sustainability of Perioperative Efficiency of Anesthesiologists.
PMID: 31406965 [PubMed]
PMCID: PMC6690616
EC Pharmacology and Toxicology
A Pilot STEM Curriculum Designed to Teach High School Students Concepts in Biochemical Engineering and Pharmacology.
PMID: 31517314 [PubMed]
PMCID: PMC6741290
EC Pharmacology and Toxicology
Toxic Mechanisms Underlying Motor Activity Changes Induced by a Mixture of Lead, Arsenic and Manganese.
PMID: 31633124 [PubMed]
PMCID: PMC6800226
EC Neurology
Research Volunteers' Attitudes Toward Chronic Fatigue Syndrome and Myalgic Encephalomyelitis.
PMID: 29662969 [PubMed]
PMCID: PMC5898812
EC Pharmacology and Toxicology
Hyperbaric Oxygen Therapy for Alzheimer's Disease.
PMID: 30215058 [PubMed]
PMCID: PMC6133268
News and Events
July Issue Release
We always feel pleasure to share our updates with you all. Here, notifying you that we have successfully released the July issue of respective journals and the latest articles can be viewed on the current issue pages.
Submission Deadline for Upcoming Issue
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