Research Article
Volume 6 Issue 8 - 2020
Isolation, Identification and Pathogenicity of Anthracnose of Grapevine and its Possible Management
Bakhat Zaman1*, Nasir Ahmed Rajput1, Hussainullah1 and Monib Ullah2
1Department of Plant Pathology, Faculty of Agriculture, University of Agriculture, Faisalabad, Pakistan
2Institute of Microbiology, Faculty of Veterinary, University of Agriculture, Faisalabad, Pakistan
*Corresponding Author: Bakhat Zaman, Department of Plant Pathology, Faculty of Agriculture, University of Agriculture, Faisalabad, Pakistan.
Received: June 13, 2020; Published: July 16, 2020




Abstract

Grape (Vitis vinifera) is the most remunerative and economically important fruit crop and has a lot of uses. It is commonly attacked by a wide range of fungus species in which the most prominent is Elsinoe ampelina which cause anthracnose disease of grapevine. An in vitro trial was conducted in the Laboratory of Mycology, Department of Plant Pathology, Agricultural University Faisalabad, Pakistan, during 2016 - 2017 in order to properly manage Anthracnose disease of grape with systemic fungicides and plant extracts. Five systemic fungicides namely Diniconazole, Thiophanate-methyl, Myclobutanil, Difenoconazole and Hexaconazole were tested at 50 ppm, 100 ppm and 200 ppm in order to check their efficacy against percent growth inhibition of Elsinoe ampelina. Hexaconazole and Myclobutanil at 200 ppm inhibited 88.33% and 86.42% radial growth respectively and found to be significantly superior to Diniconazole and Thiophanate-methyl at 200 ppm. Difenoconazole was significantly inferior against Elsinoe ampelina. Different plant extracts of neem (Azadirachta indica), onion (Allium cepa), ginger (Zingiber officinale) and garlic (Allium sativum) were evaluated at 3 different concentrations 10%, 20% and 30% against the growth inhibition of Elsinoe ampelina. Among all the plant extracts, the highest concentration of ginger (Zingiber officinale) was found to be significantly best in radial growth inhibition of Elsinoe ampelina followed by neem (Azadirachta indica), garlic (Allium sativum) and onion (Allium cepa), each plant extracts were found to be significantly different from one another. Another field experiment was also conducted in which Diniconazole, Thiophanate methyl, Myclobutanil, Difenoconazole and Hexaconazole and plant extracts namely neem (Azadirachta indica), onion (Allium cepa), ginger (Zingiber officinale) garlic (Allium sativum) and Lantana (Lantana camara) were further evaluated under greenhouse condition, among the five tested fungicides, Hexaconazole was found to be the most effective fungicide in controlling the disease incidence, for which the disease incidence was recorded very low (40.93%) followed by Myclobutanil and Diniconazole, while Difenoconazole was found to be less effective in controlling the disease incidence. Similarly among the five plant extracts, Lantana extract was found to be highly effective in controlling disease incidence, for which the disease incidence was recorded very low (38.04%) followed by ginger (Zingiber officinale) and neem (Azadirachta indica), while onion (Allium cepa) was found to be less effective in controlling disease incidence.

Keywords: Pathogenicity; Identification; Management

References

  1. Australian Wine and Brandy Corporation. “Annual Report” (2009-2010).
  2. “Food and Agriculture Organization. Govt. of Pakistan”. FAO year Book Production 51 (1997).
  3. Anderson HW. “Diseases of fruit crops”. McGraw-Hill New York (1956): 377-382.
  4. Begum F., et al. “Integrated control of seedling mortality of lentil caused by Sclerotium rolfsii”. Bangladesh Journal of Plant Pathology's 23 (2006): 60-65.
  5. Chadha KL. “Indian viticulture scenario. “International symposium on grapes production and proceesing”. Baramati India 12 (2006).
  6. Commenil P., et al. “Purification and properties of an extracellular lipase from the fungus Botrytis cinerea”. Lipids 30 (1995): 351-356.
  7. Carisse O., et al. “A model to estimate the amount of primary inoculums of Elsinoë ampelina”. Plant Disease 95 (2011):1167-1171.
  8. Chatta SK. “Biology, perpetuation and control of grape vine anthracnose caused by Sphaceloma ampelinum”. Sprinagar 33 (1992).
  9. Emmett RW., et al. “Fungicide evaluation for control of grapes vine black spot or anthracnose”. Fungicide and Nematicide Tests 36 (1981): 40.
  10. Egan MJ., et al. “Genomes, free radicals and plant cell invasion: recent developments in plant pathogenic fungi”. Current Opinion in Plant Biology 11 (2008): 367-372.
  11. Ferreira RB., et al. “Engineering grapes vine for increased resistance to fungal pathogens without compromising wine stability”. Trends in Biotechnology 22 (2004): 168-173.
  12. Gupta JS., et al. “Effect of metabolites from different host plants on conidial germination of Colletotrichum graminicola and capsici”. Geobios 8 (1981): 226-228.
  13. Hopkins DL., et al. “A greenhouse method for screening grapes vine seedlings for resistance to anthracnose”. Horticultural Science 35 (2000): 89-91.
  14. Hopkins DL., et al. “Fungicidal control of bunch grapes diseases in Florida”. Proceedings of the Florida State Horticultural Society 86 (1973): 329-333.
  15. Kars I., et al. “Necrotizing activity of five Botrytis cinerea endopolygalacturonases produced in Pichia pastoris”. The Plant Journal 43 (2005): 213-225.
  16. Mirica IIRC., et al. “Anthracnose: Compendium of Grapes Diseases”. American Phyto-pathological Society St. Paul, MN (1988): 18-19.
  17. Nene YL., et al. “Fungicides in plant disease control”. Oxford and IBH publishing Co (1993): 691.
  18. Pampanagouda B. “Studies on anthracnose of grapes caused by Sphaceloma ampelinum”. Thesis submitted to the University of Agricultural Sciences Dharwad (2000):113.
  19. Suhag LS., et al. “Third intern Symposium”. Subtrop Trop Hort Bangalore (1972): 207-208.
  20. Sastry MNL., et al. “Efficiency of some fungicides in the control of anthracnose of grapes vine”. Pesticides 23 (1989): 29-30.
  21. Sundar AR., et al. “In vitro Antagonism of Trichoderma against two Fungal Pathogens of Castor”. Indian Journal of Plant Protection 23 (1995): 152-155.
  22. Suhag LS., et al. “Epidemiology of grapes vine anthracnose caused by Sphaceloma ampelinumin”. North Indian Phytopathology 30 (1977): 460-465.
  23. Thind SK., et al. “Epidemiology of powdery mildew, downy mildew and Anthracnose diseases of grapes vine”. Kluwer Academic Publishers Netherlands I (2004): 621-638.
  24. Tanaka S., et al. “Studies on the grapes anthracnose Elsinoe ampelina cultural aspects of the fungus”. Bulletin of the Faculty of Agriculture 25 (1974): 917-946.
  25. Winkler AJ., et al. “General Viticulture second edition”. Berkley Univ. of Calif. Press (1947).
  26. Winkler AJ. “General viticulture”. Berkeley Los Angeles London: Uni. Of Calif. Press (1970).
  27. Wheeler BEJ. “An Introduction to plant diseases”. John Wiley and Sons Limited, London (1969): 301.

 

Citation: Bakhat Zaman., et al. “Isolation, Identification and Pathogenicity of Anthracnose of Grapevine and its Possible Management”. EC Agriculture 6.8 (2020): 19-28.

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