Abstract

“Zeibel 5455” is one of the hybrid varieties of colored grapes that does not contain diglycosidic forms of anthocyanins. It also provides raw material of ecologically clean grape, as chemical fertilizers and toxicants are not used during its cultivation.

The grape-seed dried up to 7 - 9% humidity by vacuum-sublimation method, was fragmented to MM-10 in a micromill on average of 50 - 100 mcm. Fraction and biologically active compounds were extracted from the grape-seed by two different methods.

In the first stage, vegetable fat was extracted by Waters Corporation's Supercritical Fluid Extractor SFE - 100-2-C10. In the second stage, fluid extraction of microdispersed micropowder of the grape-seed with ethyl alcohol was performed, namely optimal parameters of extraction were experimentally determined: pressure 100 bar, CO₂ delivery rate 7.5 kg/h. The quality of extraction was also affected by 75% ethyl alcohol in the form of solvent, the ratio of which to CO₂ was 21 - 22%.

A coupage of both extracts of Zeibel 5455 with 1:1 relationship and the concentration were made in the first stage with vacuum-rotary evaporator until the consistence of 61 - 63% dry substances and in the second stage by vacuum sublimation or lyophilized method till the consistence of 74 - 75% dry substances. Biologically active compounds of the received liquid concentrate and the antioxidant activity were estimated.

More than 81% of the fatty acids existed in the composition of the received liquid bioflavanoid concentrate is unsaturated, rich in polyphenolic complex and characterized by high antioxidant activity - 58.62% (F = 100).

Keywords: “Zeibel 5455”; Carbonic Acid; Colored Grape; Phenolic Compounds; Antioxidant Activity

References

1. Asatiani MG., et al. “Grape skin preservation”. Food industry 5 (1989): 46-48.
2. Gvinianidze TN., et al. “Storage of Wine-Making Secondary Resources as the Richest Source of Biologically Active Substances”. Proceedings of National Polytechnic University of Armenia. Yerevan (2015): 40-47.
3. HN Rajha., et al. "An Environment Friendly, Low-Cost Extraction Process of Phenolic Compounds from Grape Byproducts. Optimization by Multi-Response Surface Methodology". Food and Nutrition Sciences6 (2013): 650-659.
4. S Durmishidzea and O Khachidze. “Chemistry of grapes”. Metsniereba Publishing House, Tbilisi (1981): 192.
5. Gvinianidze T., et al. “Some Aspects of Recycling and Storage of Secondary Resources of Grape”. Bulletin of Science and Practice 7 (2019): 128-134.
6. EN Kishkovsky and IM Skurikhin. “Chemistry of Wine, food Industry”. “Food industry”, Moscow (1976).
7. Conceptual and economic rationale for the effectiveness of the cluster approach to the processing of secondary raw materials of winemaking. Access mode: www.clustermdua.
8. M Ga “Developing Technologies and Quality Control Methods of Georgian Grape Seed Bioflavonoid Liquid Extracts”. The thesis presented to obtain quality Doctor's degree, Kutaisi (2017).
9. N Gvinianidze., et al. “Polyphenolic Extracts of Red Grapes”. Agricultural Research and Technology Open Access Journal2 (2018): 555981.
10. Application and characteristics of grape seed.
11. Singleton Vernon L., et al."[14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent". Methods in Enzymology 299 (1999): 152-178. 
12. SergioGómez-Alonso. “HPLC analysis of diverse grape and wine phenolics using direct injection and multidetection by DAD and fluorescence”. Journal of Food Composition and Analysis7 (2007): 618-626.
13. Palomino., et al. “Study of polyphenols in grape berries by reversed-phase high-performance liquid chromatography”. Journal of Chromatography A 1-2(2000): 449-451.
14. Mónica Giusti and Ronald E Wrolstad. “Characterization and Measurement of Anthocyanins by UV-Visible Spectroscopy”. Current Protocols in Food Analytical Chemistry (2001): F1.2.1-F1.2.13.
15. Kammerer D., et al. “Polyphenol screening of pomace from red and white gravarieties (Vitis vinifera) by HPLC-DAD-MS/MS”. Journal of Agricultural and Food Chemistry 52.14 (2004): 4360-4367.
16. Farida Benmeziane., et al. “Determination of major anthocyanin pigments and flavonols in red grape skin of some table grape varieties (Vitis vinifera sp.) by high-performance liquid chromatography-photodiode array detection”. Vine and Wine3 (2016).
17. Mensor LL., et al. “Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method”. Phytotherapy Research 2 (2001): 127-130.
18. The state pharmacopeia of the ussr eleventh editionissue 1 general analysis methods (1987):
19. , et al. “Identification and determination of ascorbic acid, free organic acids and tannic substances in the grass of Inula L. genus species”. French-Ukrainian Journal of Chemistry 7.1 (2019): 25-33.