Research Article
Volume 6 Issue 5 - 2020
Biologically Active Compounds and Antioxidant Activity of “Zeibel 5455” Grape-Seed Superfluid Extracts
TN Gvinianidze*, EG Margvelashvili and RS Kopaliani
Akaki Tsereteli State University, Kutaisi, Georgia
*Corresponding Author: TN Gvinianidze, Akaki Tsereteli State University, Kutaisi, Georgia.
Received: March 21, 2020; Published: April 06, 2020




Abstract

“Zeibel 5455” is one of the hybrid varieties of colored grapes that does not contain diglycosidic forms of anthocyanins. It also provides raw material of ecologically clean grape, as chemical fertilizers and toxicants are not used during its cultivation.

The grape-seed dried up to 7 - 9% humidity by vacuum-sublimation method, was fragmented to MM-10 in a micromill on average of 50 - 100 mcm. Fraction and biologically active compounds were extracted from the grape-seed by two different methods.

In the first stage, vegetable fat was extracted by Waters Corporation's Supercritical Fluid Extractor SFE - 100-2-C10. In the second stage, fluid extraction of microdispersed micropowder of the grape-seed with ethyl alcohol was performed, namely optimal parameters of extraction were experimentally determined: pressure 100 bar, CO2 delivery rate 7.5 kg/h. The quality of extraction was also affected by 75% ethyl alcohol in the form of solvent, the ratio of which to CO2 was 21 - 22%.

A coupage of both extracts of Zeibel 5455 with 1:1 relationship and the concentration were made in the first stage with vacuum-rotary evaporator until the consistence of 61 - 63% dry substances and in the second stage by vacuum sublimation or lyophilized method till the consistence of 74 - 75% dry substances. Biologically active compounds of the received liquid concentrate and the antioxidant activity were estimated.

More than 81% of the fatty acids existed in the composition of the received liquid bioflavanoid concentrate is unsaturated, rich in polyphenolic complex and characterized by high antioxidant activity - 58.62% (F = 100).

Keywords:Zeibel 5455; Carbonic Acid; Colored Grape; Phenolic Compounds; Antioxidant Activity

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Citation: TN Gvinianidze., et al. “Biologically Active Compounds and Antioxidant Activity of “Zeibel 5455” Grape-Seed Superfluid Extracts”. EC Agriculture 6.5 (2020): 08-16.

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