Research Article
Volume 6 Issue 4 - 2020
Chitinase Activity of Biocontrol Fungi Isolated from the Golden Potato Cyst Nematode and their Antagonist Potential
Khadijeh Abbasi1, Robert Wick2* and Doustmorad Zafari3
1Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
2Stockbridge School of Agriculture, University of Massachusetts, Amherst
3Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
*Corresponding Author: Robert Wick, Stockbridge School of Agriculture, University of Massachusetts, Amherst.
Received: February 22, 2020; Published: March 10, 2020




Abstract

Chitin is the most abundant polymer in the nematode eggshell. Chitinases are widely distributed in fungi and play important roles in the degradation of chitin. This study aimed to screen the chitinolytic ability of fungi isolated from Globodera rostochiensis and to evaluate their biocontrol potentialOne hundred and fifty-four fungal isolates were compared for their ability to degrade chitin by the N-acetyl-glucosamine-dinitrosalicylate method. Ten isolates with high chitinase activity were selected for further characterization. Of the 10 selected isolates, three chitinase enzymes were evaluated using three substrates. Based on the results of our chitinase assay, Beauveria bassiana, Lecanicillium muscarium, Paecilomyces sp. and Trichoderma atroviridae had the highest activity. We selected these four isolates to determine the optimum pH, temperature and reaction time. Zymography was also used to demonstrate chitinase activity of the four isolates. The biocontrol potential of 10 selected isolates was assayed in water-agar in vitro and under greenhouse conditions. L. muscarium had the most potential and Fusarium solani was the least based on the number of parasitized juveniles and eggs in water-agar. Greenhouse trials showed T. atroviridae and B. bassiana had the highest dry root weight and tuber yield.

Keywords: Biocontrol; Chitinase; Enzymatic Activity; Fungi; Globodera rostochiensis

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  • Citation: Robert Wick., et al. “Chitinase Activity of Biocontrol Fungi Isolated from the Golden Potato Cyst Nematode and their Antagonist Potential”. EC Agriculture 6.4 (2020): 05-15.

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