Research Article
Volume 6 Issue 2 - 2020
Assessing Microsatellite Markers from Juglans nigra L. for Differentiation of Trees from the Walnut Hybrid Progeny Mj209xRa
Ricardo-Julian Licea-Moreno1,2*, Angela Contreras2, Elke Pita Thomas3 and Luis Gomez2,3
1Department of Biotechnology, Bosques Naturales SA, Madrid, Spain
2Center for Plant Biotechnology and Genomics (CBGP), Universidad Politecnica de Madrid, Montegancedo Campus, Madrid, Spain
3Departamento de Sistemas y Recursos Naturales, ETSI Montes, Forestal y del Medio Natural, Universidad Politecnica de Madrid, Madrid, Spain
*Corresponding Author: Ricardo-Julian Licea-Moreno, Department of Biotechnology, Bosques Naturales SA, Madrid, Spain.
Received: December 24, 2019; Published: January 24, 2020




Abstract

Timber from walnut is highly prized by its aesthetical, mechanical and physical properties. Although several varieties for fruit production have been obtained, few genotypes and progenies exist for wood production. The French hybrid progeny Mj209xRa has showed great adaptability to European conditions, and its superiority regarding other hybrids and pure species, becoming the most valuable and widely used progeny for timber production. Although the great phenotypic variability that affects seed plantations reduces their industrial exploitation, it is a suitable opportunity for the selection of outstanding genotypes. Since this progeny is formed by half-siblings, the use of solid markers, as microsatellites, might help to characterize the genetic variability and to differentiate close related individuals. Recently have been published microsatellite markers for this progeny; however, because of the great degree of kinship of Mj209xRa offspring, might be necessary more markers to differentiate individuals with a high certainty degree. Therefore, the suitability of microsatellite markers from American black walnut (Juglans nigra L.) for genetic differentiation of trees from the walnut hybrid progeny Mj209xRa was assessed. Genomic DNA from 50 trees from the hybrid progeny Mj209xRa were used. For PCR amplifications, 10 microsatellite markers were selected, and statistical analysis were performed to determine their individual and combined differentiation capacity. PCR products from all 10 loci were obtained, although for 2 of them the amplifications were non-scorable. In general, reduced polymorphisms (3 alleles/locus) as well as lower heterozygosities and combined discriminative power (PIDsib in the order of 10-2) were registered. Hence, this set of SSR markers was not suitable to differentiate putative hybrid trees from the Mj209xRa progeny. However, 3 loci can be used as a complement for other microsatellite sets, increasing thus the discriminative capacity of the existing markers.


Keywords: Timber Production; Genotyping; Genotype Identification; Simple Sequence Repeats; SSR

References

  1. Wiedenbeck J., et al. "Defining hardwood veneer log quality attributes". General Technical Report NE-313. Newtown Square, PA: US Department of Agriculture, Forest Service, Northeastern Research Station (2004): 36. 
  2. Cassens DL. "Factors affecting the quality of walnut lumber and veneer". Proceedings of the 6th Walnut Council Research Symposium. Black Walnut in a New Century. Lafayette, EUA (2004): 161-167. 
  3. Ducci F and Malvotti ME. “Recent developments in the selection and breeding programme on Juglans spp. in Italy”. Cost Action E42 Growing Valuable Broadleaved Tree Species. WG1 Workshop on Genetics Leuven, Belgium (2006). 
  4. Fady B., et al. "Walnut demonstrates strong genetic variability for adaptive and wood quality traits in a network of juvenile field tests across Europe". New Forests 25.3 (2003): 211-225.
  5. Jay-Allemand C., et al. "Walnut trees for woodland use in Mediterranean countries: current situation and prospects". FAO Nucis Newsletter 5 (1996): 10-13.
  6. Becquey J. “Les noyers a bois. Fore t prive e française”. In: Les guides du sylviculteur. Troise me Edition (1997): 144.
  7. Paris P., et al. “Primi risultati di prove comparative d’accessioni europee di noce da legno”. Atti del III Congresso Nazionale SISEF” Alberi e Foreste per il Nuovo Millennio”, Viterbo (2001): 15-18.
  8. Aleta N., et al. "Characterization of the agroforestry performance of 12 walnut (Juglans sp.) genotypes grown in two locations of Catalonia". Forest Systems 12.1 (2003): 39-50. 
  9. Clark J and Gabriel H. "Walnut hybrids in the UK: fastgrowing quality hardwoods". Quarterly Journal of Forestry 104.1 (2010): 43-46.
  10. Aleta N and Vilanova A. "El nogal hí brido". Navarra Forestal 13 (2006): 18-21. 
  11. Contreras A., et al. "New set of microsatellite markers for the walnut hybrid progeny Mj209xRa and assessment of its transferability into Juglans genus". Forest Systems 28.2 (2019): 009. 
  12. Kalinowski ST. "How many alleles per locus should be used to estimate genetic distances?" Heredity 88.1 (2002): 62. 
  13. Ross-Davis A and Woeste KE. "Microsatellite markers for Juglans cinerea L. and their utility in other Juglandaceae species". Conservation Genetics 9.2 (2008): 465-469. 
  14. Victory ER., et al. "Genetic homogeneity in Juglans nigra (Juglandaceae) at nuclear microsatellites". American Journal of Botany 93.1 (2006): 118-126. 
  15. Wagner HW and SEFC KM. “Identity 1.0”. Centre for Applied Genetics, University of Agricultural Sciences, Vienna 500 (1999).
  16. Woods JG., et al. "Genetic tagging of free-ranging black and brown bears". Wildlife Society Bulletin 27.3 (1999): 616-627. 
  17. Waits LP., et al. "Estimating the probability of identity among genotypes in natural populations: cautions and guidelines". Molecular Ecology 10.1 (2001): 249-256. 
  18. Perrier X and Jacquemoud-Collet JP. “DARwin software” (2006). 
  19. Woeste K., et al. "Thirty polymorphic nuclear microsatellite loci from black walnut". Journal of Heredity 93.1 (2002): 58-60. 
  20. Dangl GS., et al. "Characterization of 14 microsatellite markers for genetic analysis and cultivar identification of walnut". Journal of the American Society for Horticultural Science 130.3 (2005): 348-354. 
  21. Turchetto C., et al. "Genetic differentiation and hybrid identification using microsatellite markers in closely related wild species". AoB Plants 7 (2015). 
  22. Hu YH., et al. "De novo assembly and characterization of transcriptome using Illumina sequencing and development of twenty five microsatellite markers for an endemic tree Juglans hopeiensis Hu in China". Biochemical Systematics and Ecology 63 (2015): 201-211.
Citation: Ricardo-Julian Licea-Moreno., et al. “Assessing Microsatellite Markers from Juglans nigra L. for Differentiation of Trees from the Walnut Hybrid Progeny Mj209xRa”. EC Agriculture 6.2 (2020): 01-07.

PubMed Indexed Article


EC Pharmacology and Toxicology
LC-UV-MS and MS/MS Characterize Glutathione Reactivity with Different Isomers (2,2' and 2,4' vs. 4,4') of Methylene Diphenyl-Diisocyanate.

PMID: 31143884 [PubMed]

PMCID: PMC6536005


EC Pharmacology and Toxicology
Alzheimer's Pathogenesis, Metal-Mediated Redox Stress, and Potential Nanotheranostics.

PMID: 31565701 [PubMed]

PMCID: PMC6764777


EC Neurology
Differences in Rate of Cognitive Decline and Caregiver Burden between Alzheimer's Disease and Vascular Dementia: a Retrospective Study.

PMID: 27747317 [PubMed]

PMCID: PMC5065347


EC Pharmacology and Toxicology
Will Blockchain Technology Transform Healthcare and Biomedical Sciences?

PMID: 31460519 [PubMed]

PMCID: PMC6711478


EC Pharmacology and Toxicology
Is it a Prime Time for AI-powered Virtual Drug Screening?

PMID: 30215059 [PubMed]

PMCID: PMC6133253


EC Psychology and Psychiatry
Analysis of Evidence for the Combination of Pro-dopamine Regulator (KB220PAM) and Naltrexone to Prevent Opioid Use Disorder Relapse.

PMID: 30417173 [PubMed]

PMCID: PMC6226033


EC Anaesthesia
Arrest Under Anesthesia - What was the Culprit? A Case Report.

PMID: 30264037 [PubMed]

PMCID: PMC6155992


EC Orthopaedics
Distraction Implantation. A New Technique in Total Joint Arthroplasty and Direct Skeletal Attachment.

PMID: 30198026 [PubMed]

PMCID: PMC6124505


EC Pulmonology and Respiratory Medicine
Prevalence and factors associated with self-reported chronic obstructive pulmonary disease among adults aged 40-79: the National Health and Nutrition Examination Survey (NHANES) 2007-2012.

PMID: 30294723 [PubMed]

PMCID: PMC6169793


EC Dental Science
Important Dental Fiber-Reinforced Composite Molding Compound Breakthroughs

PMID: 29285526 [PubMed]

PMCID: PMC5743211


EC Microbiology
Prevalence of Intestinal Parasites Among HIV Infected and HIV Uninfected Patients Treated at the 1o De Maio Health Centre in Maputo, Mozambique

PMID: 29911204 [PubMed]

PMCID: PMC5999047


EC Microbiology
Macrophages and the Viral Dissemination Super Highway

PMID: 26949751 [PubMed]

PMCID: PMC4774560


EC Microbiology
The Microbiome, Antibiotics, and Health of the Pediatric Population.

PMID: 27390782 [PubMed]

PMCID: PMC4933318


EC Microbiology
Reactive Oxygen Species in HIV Infection

PMID: 28580453 [PubMed]

PMCID: PMC5450819


EC Microbiology
A Review of the CD4 T Cell Contribution to Lung Infection, Inflammation and Repair with a Focus on Wheeze and Asthma in the Pediatric Population

PMID: 26280024 [PubMed]

PMCID: PMC4533840


EC Neurology
Identifying Key Symptoms Differentiating Myalgic Encephalomyelitis and Chronic Fatigue Syndrome from Multiple Sclerosis

PMID: 28066845 [PubMed]

PMCID: PMC5214344


EC Pharmacology and Toxicology
Paradigm Shift is the Normal State of Pharmacology

PMID: 28936490 [PubMed]

PMCID: PMC5604476


EC Neurology
Examining those Meeting IOM Criteria Versus IOM Plus Fibromyalgia

PMID: 28713879 [PubMed]

PMCID: PMC5510658


EC Neurology
Unilateral Frontosphenoid Craniosynostosis: Case Report and a Review of the Literature

PMID: 28133641 [PubMed]

PMCID: PMC5267489


EC Ophthalmology
OCT-Angiography for Non-Invasive Monitoring of Neuronal and Vascular Structure in Mouse Retina: Implication for Characterization of Retinal Neurovascular Coupling

PMID: 29333536 [PubMed]

PMCID: PMC5766278


EC Neurology
Longer Duration of Downslope Treadmill Walking Induces Depression of H-Reflexes Measured during Standing and Walking.

PMID: 31032493 [PubMed]

PMCID: PMC6483108


EC Microbiology
Onchocerciasis in Mozambique: An Unknown Condition for Health Professionals.

PMID: 30957099 [PubMed]

PMCID: PMC6448571


EC Nutrition
Food Insecurity among Households with and without Podoconiosis in East and West Gojjam, Ethiopia.

PMID: 30101228 [PubMed]

PMCID: PMC6086333


EC Ophthalmology
REVIEW. +2 to +3 D. Reading Glasses to Prevent Myopia.

PMID: 31080964 [PubMed]

PMCID: PMC6508883


EC Gynaecology
Biomechanical Mapping of the Female Pelvic Floor: Uterine Prolapse Versus Normal Conditions.

PMID: 31093608 [PubMed]

PMCID: PMC6513001


EC Dental Science
Fiber-Reinforced Composites: A Breakthrough in Practical Clinical Applications with Advanced Wear Resistance for Dental Materials.

PMID: 31552397 [PubMed]

PMCID: PMC6758937


EC Microbiology
Neurocysticercosis in Child Bearing Women: An Overlooked Condition in Mozambique and a Potentially Missed Diagnosis in Women Presenting with Eclampsia.

PMID: 31681909 [PubMed]

PMCID: PMC6824723


EC Microbiology
Molecular Detection of Leptospira spp. in Rodents Trapped in the Mozambique Island City, Nampula Province, Mozambique.

PMID: 31681910 [PubMed]

PMCID: PMC6824726


EC Neurology
Endoplasmic Reticulum-Mitochondrial Cross-Talk in Neurodegenerative and Eye Diseases.

PMID: 31528859 [PubMed]

PMCID: PMC6746603


EC Psychology and Psychiatry
Can Chronic Consumption of Caffeine by Increasing D2/D3 Receptors Offer Benefit to Carriers of the DRD2 A1 Allele in Cocaine Abuse?

PMID: 31276119 [PubMed]

PMCID: PMC6604646


EC Anaesthesia
Real Time Locating Systems and sustainability of Perioperative Efficiency of Anesthesiologists.

PMID: 31406965 [PubMed]

PMCID: PMC6690616


EC Pharmacology and Toxicology
A Pilot STEM Curriculum Designed to Teach High School Students Concepts in Biochemical Engineering and Pharmacology.

PMID: 31517314 [PubMed]

PMCID: PMC6741290


EC Pharmacology and Toxicology
Toxic Mechanisms Underlying Motor Activity Changes Induced by a Mixture of Lead, Arsenic and Manganese.

PMID: 31633124 [PubMed]

PMCID: PMC6800226


EC Neurology
Research Volunteers' Attitudes Toward Chronic Fatigue Syndrome and Myalgic Encephalomyelitis.

PMID: 29662969 [PubMed]

PMCID: PMC5898812


EC Pharmacology and Toxicology
Hyperbaric Oxygen Therapy for Alzheimer's Disease.

PMID: 30215058 [PubMed]

PMCID: PMC6133268


News and Events


June Issue Release

We always feel pleasure to share our updates with you all. Here, notifying you that we have successfully released the June issue of respective journals and the latest articles can be viewed on the current issue pages.

Submission Deadline for Upcoming Issue

ECronicon delightfully welcomes all the authors around the globe for effective collaboration with an article submission for the upcoming issue of respective journals. Submissions are accepted on/before July 05, 2022.

Certificate of Publication

ECronicon honors with a "Publication Certificate" to the corresponding author by including the names of co-authors as a token of appreciation for publishing the work with our respective journals.

Best Article of the Issue

Editors of respective journals will always be very much interested in electing one Best Article after each issue release. The authors of the selected article will be honored with a "Best Article of the Issue" certificate.

Certifying for Review

ECronicon certifies the Editors for their first review done towards the assigned article of the respective journals.

Latest Articles

The latest articles will be updated immediately on the articles in press page of the respective journals.